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Literature summary extracted from

  • de Alcantara, P.H.; Martim, L.; Silva, C.O.; Dietrich, S.M.; Buckeridge, M.S.
    Purification of a beta-galactosidase from cotyledons of Hymenaea courbaril L. (Leguminosae). Enzyme properties and biological function (2006), Plant Physiol. Biochem., 44, 619-627.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 D-galactose competitive product inhibition Hymenaea courbaril

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 xyloglucan oligosaccharides + H2O Hymenaea courbaril the enzyme hcbetagal is involved in storage xyloglucan mobilisation during seedling development, hcbetagal might be one of the biochemical control points in xyloglucan catabolism in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Hymenaea courbaril
-
 a legume tree from the Neotropical region of the world
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 native xyloglucan-specific enzyme 13.5fold from cotyledon of developing seedlings by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Hymenaea courbaril

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 cotyledon of developing seedlings Hymenaea courbaril
-
3.2.1.23 flower
-
 Hymenaea courbaril
-
3.2.1.23 fruit ripening Hymenaea courbaril
-
3.2.1.23 leaf
-
 Hymenaea courbaril
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3.2.1.23 seedling
-
 Hymenaea courbaril
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 0.581
-
 purified enzyme Hymenaea courbaril

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O low activity Hymenaea courbaril 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Hymenaea courbaril 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-glucopyranoside + H2O low activity Hymenaea courbaril 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Hymenaea courbaril D-glucose + D-galactose
-
 ?
3.2.1.23 additional information no activity with xylose- or mannose-containing substrates Hymenaea courbaril ?
-
 ?
3.2.1.23 xyloglucan oligosaccharides + H2O the enzyme hcbetagal is involved in storage xyloglucan mobilisation during seedling development, hcbetagal might be one of the biochemical control points in xyloglucan catabolism in vivo Hymenaea courbaril ?
-
 ?
3.2.1.23 xyloglucan oligosaccharides + H2O the enzyme hcbetagal is specific for xyloglucan oligosaccharides, but does not attack the polymer Hymenaea courbaril ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 ? x * 52000-62000 Hymenaea courbaril

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 hcbetagal
-
 Hymenaea courbaril
3.2.1.23 XG-specific beta-galactosidase
-
 Hymenaea courbaril

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
-
 Hymenaea courbaril

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 3 4
-
 Hymenaea courbaril

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 3.7
-
 D-galactose pH 3.0-4.0, 30°C Hymenaea courbaril