Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Le Roy, K.; Lammens, W.; Verhaest, M.; De Coninck, B.; Rabijns, A.; Van Laere, A.; Van den Ende, W.
    Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase (2007), Plant Physiol., 145, 616-625.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 expressed in Pichia pastoris Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.80 additional information a D239A mutant of cell wall invertase 1 acts as a 1-FEH, preferentially degrading 1-kestose Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 sucrose
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.80 0.6
-
 1-kestose in 50 mM sodium acetate buffer, pH 5.0, at 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Arabidopsis thaliana Q67XZ3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 1-kestose + H2O
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 1-FEH
-
 Arabidopsis thaliana
3.2.1.80 1-KEH
-
 Arabidopsis thaliana
3.2.1.80 1-kestose exohydrolase
-
 Arabidopsis thaliana
3.2.1.80 AtcwINV1 D239A mutant
-
 Arabidopsis thaliana
3.2.1.80 fructan 1-exohydrolase
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.80 21
-
 1-kestose in 50 mM sodium acetate buffer, pH 5.0, at 30°C Arabidopsis thaliana