Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Strasser, R.; Bondili, J.S.; Schoberer, J.; Svoboda, B.; Liebminger, E.; Gloessl, J.; Altmann, F.; Steinkellner, H.; Mach, L.
    Enzymatic properties and subcellular localization of Arabidopsis beta-N-acetylhexosaminidases (2007), Plant Physiol., 145, 5-16.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in Sf21 cells. HEXO fluorescent protein fusions transiently expressed in Nicotiana benthamiana Arabidopsis thaliana
3.2.1.52 HEXO1 lacking its 32 N-terminal amino acids is cloned into baculovirus transfer vector pVTBac-His-1 and is expressed in Sf21. HEXO fluorescent protein fusions transiently expressed in Nicotiana benthamiana Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.7
-
 p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 Arabidopsis thaliana
3.2.1.52 1.2
-
 p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 Arabidopsis thaliana
3.2.1.52 2.2
-
 p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 plasma membrane
-
 Arabidopsis thaliana 5886
-
3.2.1.52 vacuole HEXO1 participates in N-glycan trimming in the vacuole Arabidopsis thaliana 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 61000
-
 x * 61000, calculated from sequence Arabidopsis thaliana
3.2.1.52 61800
-
 x * 61800, calculated from sequence Arabidopsis thaliana
3.2.1.52 64000
-
 x * 64000, SDS-PAGE Arabidopsis thaliana
3.2.1.52 65000
-
 x * 65000, SDS-PAGE Arabidopsis thaliana
3.2.1.52 65600
-
 x * 65600, calculated from sequence Arabidopsis thaliana
3.2.1.52 68000
-
 x * 68000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 additional information Arabidopsis thaliana HEXO1 participates in N-glycan trimming in the vacuole ?
-
 ?
3.2.1.52 additional information Arabidopsis thaliana HEXO2 is responsible for the processing of N-glycans present on secretory glycoproteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Arabidopsis thaliana A7WM73
-
-
3.2.1.52 Arabidopsis thaliana Q8L7S6
-
-
3.2.1.52 Arabidopsis thaliana Q9SYK0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Arabidopsis thaliana 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Arabidopsis thaliana 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.52 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Arabidopsis thaliana 4-methylumbelliferone + 6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.52 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
 Arabidopsis thaliana 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-galactopyranose
-
 ?
3.2.1.52 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Arabidopsis thaliana 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.52 additional information HEXO1 participates in N-glycan trimming in the vacuole Arabidopsis thaliana ?
-
 ?
3.2.1.52 additional information HEXO2 is responsible for the processing of N-glycans present on secretory glycoproteins Arabidopsis thaliana ?
-
 ?
3.2.1.52 additional information HEXO1 is capable of removing terminal GlcNAc residues from the alpha1,3- and alpha1,6-mannosyl branches of biantennary N-glycans without any strict branch preference with N-glycan substrates. HEXO1 displayed a much higher specific activity than HEXO2 and HEXO3 Arabidopsis thaliana ?
-
 ?
3.2.1.52 p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
 Arabidopsis thaliana p-nitrophenol + 2-acetamido-2-deoxy-beta-D-galactopyranose
-
 ?
3.2.1.52 p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Arabidopsis thaliana p-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.52 pyridylaminated chitobiose + H2O
-
 Arabidopsis thaliana ?
-
 ?
3.2.1.52 pyridylaminated chitotriose + H2O
-
 Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 65000, SDS-PAGE Arabidopsis thaliana
3.2.1.52 ? x * 64000, SDS-PAGE Arabidopsis thaliana
3.2.1.52 ? x * 68000, SDS-PAGE Arabidopsis thaliana
3.2.1.52 ? x * 61000, calculated from sequence Arabidopsis thaliana
3.2.1.52 ? x * 61800, calculated from sequence Arabidopsis thaliana
3.2.1.52 ? x * 65600, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 At1g65600/F5I14_13
-
 Arabidopsis thaliana
3.2.1.52 F3F20.4 protein
-
 Arabidopsis thaliana
3.2.1.52 hEXO1
-
 Arabidopsis thaliana
3.2.1.52 HEXO2
-
 Arabidopsis thaliana
3.2.1.52 HEXO3
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4
-
 hydrolysis p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Arabidopsis thaliana
3.2.1.52 5
-
 hydrolysis p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Arabidopsis thaliana