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Literature summary extracted from

  • Clauss, K.; Baumert, A.; Nimtz, M.; Milkowski, C.; Strack, D.
    Role of a GDSL lipase-like protein as sinapine esterase in Brassicaceae (2008), Plant J., 53, 802-813.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.49 expressed in Nicotiana benthamiana leaves Brassica napus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.49 Phenylmethylsulfonylfluoride 50% inhibition at about 8 mM PMSF Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.49 0.0026
-
 Sinapoylcholine 30°C, pH 8.5 Brassica napus
3.1.1.49 0.011
-
 Cinnamoylcholine 30°C, pH 8.5 Brassica napus
3.1.1.49 0.101
-
 3-phenylpropionylcholine 30°C, pH 8.5 Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.49 44000
-
 1 * 44000, SDS-PAGE Brassica napus
3.1.1.49 47000
-
 gel filtration Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.49 sinapoylcholine + H2O Brassica napus role for the GDSL lipase-like BnSCE3/BnLIP2 as a sinapine esterase, catalyzing hydrolysis of sinapine during seed germination, leading, via 1-O-sinapoyl-beta-glucose, to sinapoyl-L-malate in the seedlings sinapic acid + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.49 Brassica napus Q3ZFI4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.49
-
 Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.49 seedling
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.49 8.3
-
-
 Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.49 3-phenylpropionylcholine + H2O
-
 Brassica napus 3-phenylpropionate + choline
-
 ?
3.1.1.49 4-coumaroylcholine + H2O 20% of activity with sinapoylcholine Brassica napus coumaric acid + choline
-
 ?
3.1.1.49 benzoylcholine + H2O 18% of activity with sinapoylcholine Brassica napus benzoate + choline
-
 ?
3.1.1.49 caffeoylcholine 25% of activity with sinapoylcholine Brassica napus caffeic acid + choline
-
 ?
3.1.1.49 cinnamoylcholine + H2O 51% of activity with sinapoylcholine Brassica napus cinnamic acid + choline
-
 ?
3.1.1.49 feruloylcholine + H2O 37% of activity with sinapoylcholine Brassica napus ferulic acid + choline
-
 ?
3.1.1.49 sinapoylcholine + H2O
-
 Brassica napus sinapic acid + choline
-
 ?
3.1.1.49 sinapoylcholine + H2O role for the GDSL lipase-like BnSCE3/BnLIP2 as a sinapine esterase, catalyzing hydrolysis of sinapine during seed germination, leading, via 1-O-sinapoyl-beta-glucose, to sinapoyl-L-malate in the seedlings Brassica napus sinapic acid + choline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.49 monomer 1 * 44000, SDS-PAGE Brassica napus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.49 BnLIP2
-
 Brassica napus
3.1.1.49 BnSCE3
-
 Brassica napus
3.1.1.49 BnSCE3/BnLIP2
-
 Brassica napus