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Literature summary extracted from

  • Qian, W.; Yu, C.; Qin, H.; Liu, X.; Zhang, A.; Johansen, I.E.; Wang, D.
    Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana (2007), Plant J., 49, 399-413.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.8 D-Glucose 1,6-bisphosphate in presence of 0.02 and 0.2 mM mannose 1-phosphate substrate, half-maximal stimulation of recombinant enzyme at 0.004 and 0.008 mM D-glucose 1,6-bisphosphate Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.8
-
 Nicotiana benthamiana
5.4.2.8 expression in Escherichia coli with His-tag, expression as green fluorescent protein fusion protein in Arabidospsis thaliana Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.8 additional information expression as green fluorescent protein fusion protein in Arabidospsis thaliana increases ascorbic acid content by 25-33% Arabidopsis thaliana
5.4.2.8 additional information reducing enzyme expression level through virus-induced gene silencing causes substantial decrease in ascorbic acid content in leaves. Raising the expression level leads to 20-50% increase in ascorbic acid content Nicotiana benthamiana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.8 0.0297
-
 D-mannose 1-phosphate 30°C, pH 7.5 Arabidopsis thaliana
5.4.2.8 0.0654
-
 D-glucose 1-phosphate 30°C, pH 7.5 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.8 27700
-
 x * 27700, calculated and MALDI-TOF Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.8 Arabidopsis thaliana O80840
-
-
5.4.2.8 Nicotiana benthamiana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.8 additional information enzyme is constitutively expressed in both vegetative and reproductive organs Arabidopsis thaliana
-
5.4.2.8 additional information enzyme is constitutively expressed in both vegetative and reproductive organs Nicotiana benthamiana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.8 D-glucose 1-phosphate reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate Arabidopsis thaliana D-glucose 6-phosphate
-
 ?
5.4.2.8 D-glucose 1-phosphate
-
 Nicotiana benthamiana D-glucose 6-phosphate
-
 ?
5.4.2.8 D-Mannose 1-phosphate reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate Arabidopsis thaliana D-Mannose 6-phosphate
-
 r
5.4.2.8 D-Mannose 1-phosphate
-
 Nicotiana benthamiana D-Mannose 6-phosphate
-
 r
5.4.2.8 D-mannose 6-phosphate
-
 Arabidopsis thaliana D-mannose 1-phosphate
-
 r
5.4.2.8 D-mannose 6-phosphate
-
 Nicotiana benthamiana D-mannose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.4.2.8 ? x * 27700, calculated and MALDI-TOF Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.2.8 30
-
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.8 7.1
-
 95% of maximum activity Arabidopsis thaliana
5.4.2.8 7.5
-
-
 Arabidopsis thaliana