Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Han, S.E.; Seo, Y.S.; Kim, D.; Sung, S.K.; Kim, W.T.
    Expression of MdCAS1 and MdCAS2, encoding apple beta-cyanoalanine synthase homologs, is concomitantly induced during ripening and implicates MdCASs in the possible role of the cyanide detoxification in Fuji apple (Malus domestica Borkh.) fruits (2007), Plant Cell Rep., 26, 1321-1331.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.9 ethylene
-
 Malus domestica
4.4.1.9 additional information CAS1 and CAS2 mRNAs are coordinately induced during the ripening process of apple fruits, enzyme activity is also enhanced as the fruit ripens, although this increase is not as dramatic as the mRNA induction pattern, genes also activated effectively by mechanical wounding Malus domestica

Application

EC Number Application Comment Organism
4.4.1.9 agriculture CAS1 and CAS2 encode Fuji apple beta-CAS homologs, overall nucleotide sequence identity is 89%, whereas coding regions are 93% identical at the nucleotide level and 94% identical at the amino acid level, CAS may play a role in cyanide detoxification in ripening apple fruits Malus domestica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.9 full-length MdCAS2 cDNA clones introduced into plasmid pMAL-c2X, expressed in Escherichia coli BL21 (DE3) Malus domestica
4.4.1.9 full-length pMdCAS1 cDNA clones introduced into plasmid pMAL-c2X, expressed in Escherichia coli BL21 (DE3) Malus domestica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.9 40500
-
 sequence analysis Malus domestica
4.4.1.9 40900
-
 sequence analysis Malus domestica

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.9 Malus domestica Q1KLZ1 cv. Fuji
-
4.4.1.9 Malus domestica Q1KLZ2 cv. Fuji
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.9
-
 Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.9 fruit
-
 Malus domestica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.9 L-cysteine + HCN
-
 Malus domestica H2S + L-3-cyanoalanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.9 beta-cyanoalanine synthase
-
 Malus domestica
4.4.1.9 CAS1
-
 Malus domestica
4.4.1.9 CAS2
-
 Malus domestica