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Literature summary extracted from
- Plants utilize isoprene emission as a thermotolerance mechanism (2007), Plant Cell Physiol., 48, 1254-1262.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.27 | the plasmid pGWB2-PaIspS is introduced via Agrobacterium tumefaciens into Arabidopsis plants | Populus alba |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.3.27 | plastid | - |
Populus alba | 9536 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.27 | Mg2+ | - |
Populus alba |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.27 | dimethylallyl diphosphate | Populus alba | - |
isoprene + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.27 | Populus alba | Q50L36 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.3.27 | leaf | - |
Populus alba | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.27 | 1500 | - |
cell free exract from leaf transformants | Populus alba |
| 4.2.3.27 | 4967 | - |
native level in Populus alba leaves | Populus alba |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.27 | dimethylallyl diphosphate | - |
Populus alba | isoprene + diphosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.3.27 | isoprene synthase | - |
Populus alba |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.27 | 40 | - |
activity assay, volatile compounds of the reaction are trapped with Solid-Phase Micro Extraction fiber, which is directly injected to a gas-liqiud chromatograph | Populus alba |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.27 | 8 | - |
activity assay | Populus alba |
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Release 2023.1 (January 2023)
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