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Literature summary extracted from

  • Sawai, S.; Akashi, T.; Sakurai, N.; Suzuki, H.; Shibata, D.; Ayabe, S.; Aoki, T.
    Plant lanosterol synthase: divergence of the sterol and triterpene biosynthetic pathways in eukaryotes (2006), Plant Cell Physiol., 47, 673-677.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.7 N478H/V482I change of amino acids crucial for specificity to the cycloartenol synthase type. Mutant produces 4% lanosterol, 83% parkeol, and 13% cycloartenol from substrate (S)-2,3-epoxysqualene Lotus japonicus
5.4.99.8 additional information mutation N478H/V482I in lanosterol synthase is a change of amino acids crucial for lanosterol specificity to the cycloartenol synthase type. Mutant produces 4% lanosterol, 83% parkeol, and 13% cycloartenol from substrate (S)-2,3-epoxysqualene, while wild-type lanosterol synthase produces only lanosterol Lotus japonicus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.7 Lotus japonicus
-
-
-
5.4.99.8 Lotus japonicus
-
-
-
5.4.99.47 Lotus japonicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.7 (S)-2,3-epoxysqualene
-
 Lotus japonicus lanosterol synthesis of about 0.1% parkeol as byproduct ?
5.4.99.47 (S)-2,3-oxidosqualene
-
 Lotus japonicus parkeol + cycloartenol + lanosterol parkeol is the main product of mutant N478H/V482I ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.47 oxidosqualene cyclase 7 mutant N478H/V482I
-
 Lotus japonicus