Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Saint-Jore-Dupas, C.; Nebenfuehr, A.; Boulaflous, A.; Follet-Gueye, M.L.; Plasson, C.; Hawes, C.; Driouich, A.; Faye, L.; Gomord, V.
    Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathway (2006), Plant Cell, 18, 3182-3200.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.106 full-length Arabidopsis GCSI fused to GFP, stable expression in tobacco BY-2 cells Arabidopsis thaliana
3.2.1.113 GFP-fusions are transferred into Agrobacterium tumefaciens by heat shock Nicotiana tabacum
3.2.1.113 GFP-fusions are transferred into Agrobacterium tumefaciens by heat shock Arabidopsis thaliana
3.2.1.113 GFP-fusions are transferred into Agrobacterium tumefaciens by heat shock Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.106 additional information removal of the first 11 amino acids of the GCSI cytoplasmic tail results in relocalization from the endoplasmic reticulum to the Golgi apparatus Arabidopsis thaliana
3.2.1.113 additional information ManI fusion constructs are derived from the full-length GFP fusion Nicotiana tabacum
3.2.1.113 additional information ManI fusion constructs are derived from the full-length GFP fusion Arabidopsis thaliana
3.2.1.113 additional information ManI fusion constructs are derived from the full-length GFP fusion Solanum lycopersicum
3.2.1.113 additional information ManI fusion constructs are derived from the full-length GFP fusion Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 additional information cycloheximide, protein synthesis inhibitor. After a 2 h treatment with the protein synthesis inhibitor cycloheximide, ER and Golgi labeling observed with ManI-GFP fusion remains unchanged, showing that the steady state localization of ManI-GFP is the Golgi and the ER Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.106 endoplasmic reticulum with cytoplasmic tail, which is crucial for endoplasmic reticulum retention, thus there is a targeting mechanism relying on protein-protein interaction Arabidopsis thaliana 5783
-
3.2.1.106 membrane endoplasmic reticulum resident membrane protein, the 18-amino acid long transmembrane domain of GCSI is not sufficient to target this glycosidase in the endoplasmic reticulum membrane Arabidopsis thaliana 16020
-
3.2.1.113 endoplasmic reticulum
-
 Nicotiana tabacum 5783
-
3.2.1.113 endoplasmic reticulum
-
 Arabidopsis thaliana 5783
-
3.2.1.113 endoplasmic reticulum
-
 Solanum lycopersicum 5783
-
3.2.1.113 endoplasmic reticulum
-
 Glycine max 5783
-
3.2.1.113 Golgi apparatus different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes Nicotiana tabacum 5794
-
3.2.1.113 Golgi apparatus different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes Arabidopsis thaliana 5794
-
3.2.1.113 Golgi apparatus different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes Solanum lycopersicum 5794
-
3.2.1.113 Golgi apparatus different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes Glycine max 5794
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Arabidopsis thaliana
-
-
-
3.2.1.113 Arabidopsis thaliana
-
-
-
3.2.1.113 Glycine max Q9SEH8
-
-
3.2.1.113 Nicotiana tabacum
-
-
-
3.2.1.113 Solanum lycopersicum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.113 TBY-2 cell
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O
-
 Nicotiana tabacum alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose
-
 ?
3.2.1.113 alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O
-
 Arabidopsis thaliana alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose
-
 ?
3.2.1.113 alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O
-
 Solanum lycopersicum alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose
-
 ?
3.2.1.113 alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O
-
 Glycine max alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.106 GCSI
-
 Arabidopsis thaliana
3.2.1.106 glucosidase I
-
 Arabidopsis thaliana
3.2.1.113 alpha-1,2 mannosidase I
-
 Nicotiana tabacum
3.2.1.113 alpha-1,2 mannosidase I
-
 Arabidopsis thaliana
3.2.1.113 alpha-1,2 mannosidase I
-
 Solanum lycopersicum
3.2.1.113 alpha-1,2 mannosidase I
-
 Glycine max
3.2.1.113 ManI
-
 Nicotiana tabacum
3.2.1.113 ManI
-
 Arabidopsis thaliana
3.2.1.113 ManI
-
 Solanum lycopersicum
3.2.1.113 ManI
-
 Glycine max