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Literature summary extracted from
- Retention of a bean phaseolin/maize gamma-zein fusion in the endoplasmic reticulum depends on disulfide bond formation (2006), Plant Cell, 18, 2608-2621.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.16.5 | expressed in Nicotiana tabacum as a fusion protein | Phaseolus vulgaris |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | T343F | the mutant accumulates in the microsomal fraction of protoplasts treated with 2-mercaptoethanol and is for the most part susceptible to digestion by endoglycosidase H | Phaseolus vulgaris |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.16.5 | additional information | 20 mM 2-mercaptoethanol strongly inhibits the traffic of phaseolin, causing mainly accumulation in the endoplasmic reticulum or cis-Golgi cisternae, the inhibitory effect is more marked on the vacuolar sorting machinery | Phaseolus vulgaris |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.16.5 | vacuole | vacuolar storage protein | Phaseolus vulgaris | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 45000 | - |
3 * 45000, SDS-PAGE | Phaseolus vulgaris |
| 3.4.16.5 | 135000 | - |
SDS-PAGE | Phaseolus vulgaris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.16.5 | Phaseolus vulgaris | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | additional information | fusion with the N-terminal half of the Zea mays prolamin gamma-zein forms endoplasmic reticulum-located protein bodies | Phaseolus vulgaris |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.16.5 | seed | - |
Phaseolus vulgaris | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.16.5 | benzyloxycarbonyl-Glu-Phe + H2O | - |
Phaseolus vulgaris | benzyloxycarbonyl-Glu + Phe | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | trimer | 3 * 45000, SDS-PAGE | Phaseolus vulgaris |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | Phaseolin | - |
Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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