Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Escamilla-Trevino, L.L.; Chen, W.; Card, M.L.; Shih, M.C.; Cheng, C.L.; Poulton, J.E.
    Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides (2006), Phytochemistry, 67, 1651-1660.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.126 cDNAs encoding BGLU45 and BGLU46 expressed in Pichia pastoris Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.126 2.2
-
 p-coumaryl beta-D-glucoside BGLU46 Arabidopsis thaliana
3.2.1.126 5.1
-
 syringin BGLU45 Arabidopsis thaliana
3.2.1.126 7
-
 coniferin BGLU45 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.126 Arabidopsis thaliana
-
 ecotype Columbia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.126 to apparent homogeneity by ammonium sulfate fractionation and hydrophobic interaction chromatography, BGLU45 8.8fold and BGLU46 13.7fold purified Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.126 additional information BGLU45 and BGLU46 absent from rosette leaves or flowers Arabidopsis thaliana
-
3.2.1.126 root BGLU46 Arabidopsis thaliana
-
3.2.1.126 silique BGLU45 Arabidopsis thaliana
-
3.2.1.126 stem BGLU45 and BGLU46 display increasing levels of expression from apex to base, matching the known increase in lignification Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.126 4.1
-
 BGLU45, crude extract Arabidopsis thaliana
3.2.1.126 35.9
-
 BGLU45, purified enzyme Arabidopsis thaliana
3.2.1.126 43.8
-
 BGLU46, crude extract Arabidopsis thaliana
3.2.1.126 599
-
 BGLU46, purified enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.126 (R)-amygdalin
-
 Arabidopsis thaliana ?
-
 ?
3.2.1.126 4-methylumbelliferyl-beta-D-glucoside 9% activity with BGLU46 Arabidopsis thaliana 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.126 arbutin 6% activity with BGLU46 Arabidopsis thaliana benzene-1,4-diol + beta-D-glucose
-
 ?
3.2.1.126 coniferin 87% activity with BGLU45 and 8% activity with BGLU46 Arabidopsis thaliana beta-D-glucose + coniferyl aldehyde
-
 ?
3.2.1.126 additional information no activity with sinigrin, methyl-beta-D-glucoside, D(+)cellobiose, beta-gentiobiose, D(+)maltose, sucrose, p-nitrophenyl-1-thio-beta-D-glucopyranoside, p-nitrophenyl-alpha-D-glucopyranoside, p-nitrophenyl-N-acetyl-beta-D-glucosaminide, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-L-fucopyranoside and p-nitrophenyl-beta-L-arabinopyranoside Arabidopsis thaliana ?
-
 ?
3.2.1.126 o-nitrophenyl-beta-D-glucopyranoside most rapidly hydrolysed by BGLU45 and BGLU46 with relative rates of 619% and 449%, respectively, of the rate of p-nitrophenyl-beta-D-glucoside degradation Arabidopsis thaliana o-nitrophenol + beta-D-glucose
-
 ?
3.2.1.126 p-coumaryl beta-D-glucoside 6.9% activity with BGLU45 and 71% activity with BGLU46 Arabidopsis thaliana p-coumaric acid + beta-D-glucose
-
 ?
3.2.1.126 p-nitrophenyl-beta-D-galactopyranoside 0.4% activity with BGLU45 and 21% activity with BGLU46 Arabidopsis thaliana p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.126 p-nitrophenyl-beta-D-glucopyranoside 100% activity with BGLU45 and BGLU46 Arabidopsis thaliana p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.126 phenyl-beta-D-glucoside 62% activity with BGLU46 Arabidopsis thaliana phenol + beta-D-glucose
-
 ?
3.2.1.126 salicin 100% activity with BGLU46 Arabidopsis thaliana beta-D-glucose + salicyl alcohol
-
 ?
3.2.1.126 syringin + H2O 100% activity with BGLU45 and 6% activity with BGLU46 Arabidopsis thaliana D-glucose + sinapyl alcohol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.126 beta-glucosidase
-
 Arabidopsis thaliana
3.2.1.126 BGLU45
-
 Arabidopsis thaliana
3.2.1.126 BGLU46
-
 Arabidopsis thaliana