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Literature summary extracted from

  • Stoeckigt, J.; Panjikar, S.; Ruppert, M.; Barleben, L.; Ma, X.; Loris, E.; Hill, M.
    The molecular architecture of major enzymes from ajmaline biosynthetic pathway (2007), Phytochem. Rev., 6, 15-34.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.160 expression in Escherichia coli Rauvolfia serpentina
3.2.1.125 for heterologous expression, RG cDNA cloned into the pSE280 vector. For optimum expression, RG cDNA cloned into the pQE-2 vector and expressed in Escherichia coli strain M15 Rauvolfia serpentina

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.160 using the hanging-drop vapour-diffusion method Rauvolfia serpentina
3.2.1.125 by the hanging-drop vapour diffusion technique at 20°C. Crystals reach a maximum dimension of about 0.2 * 0.15 * 0.05 mm, belong to space group I222 and diffract to 2.30 A resolution with unit-cell parameters of a = 102.8 A, b = 127.3 A and c = 215.8 A Rauvolfia serpentina

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.160 D164A reduction in enzyme activity Rauvolfia serpentina
2.3.1.160 D32A reduction in enzyme activity Rauvolfia serpentina
2.3.1.160 D362A reduction in enzyme activity Rauvolfia serpentina
2.3.1.160 H160A reduction in enzyme activity Rauvolfia serpentina
2.3.1.160 S29A reduction in enzyme activity Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.125 1.3
-
 Raucaffricine
-
 Rauvolfia serpentina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.125 60930
-
 sequence analysis Rauvolfia serpentina
3.2.1.125 61000
-
 isolated from cell suspension Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.160 Rauvolfia serpentina
-
-
-
3.2.1.125 Rauvolfia serpentina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.160 of the recombinant protein by his-tag affinity column chromatography Rauvolfia serpentina
3.2.1.125 1200fold, by Ni-NTA column Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.160 16-epi-vellosimine + acetyl-CoA VS responsible for generation of the basic carbon skeleton of the target compound ajmaline, His160 and Asp164 are the most important amino acids for the catalytic process, His-X-X-X-Asp sequence together with Asp-Phe-Gly-Trp-Gly are highly conserved motifs in acyltransferases Rauvolfia serpentina vinorine + CoA
-
 ?
3.2.1.125 1,2-dihydroraucaffricine + H2O
-
 Rauvolfia serpentina 2beta(R)-1,2-dihydrovomilenine + D-glucose
-
 ?
3.2.1.125 raucaffricine + H2O best substrate Rauvolfia serpentina D-glucose + vomilenine
-
 ?
3.2.1.125 strictosidine + H2O
-
 Rauvolfia serpentina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.125 dimer crystallography Rauvolfia serpentina
3.2.1.125 monomer in solution Rauvolfia serpentina

Synonyms

EC Number Synonyms Comment Organism
2.3.1.160 vinorine synthase a member of the BAHD enzyme super-family, benzylalcohol acetyl-, anthocyanin-O-hydroxy-cinnamoyl-, anthranilate-N-hydroxy-cinnamoyl-benzoyl- and deacetylvindoline acetyl transferase, involved in synthesis of ajmaline Rauvolfia serpentina
2.3.1.160 VS
-
 Rauvolfia serpentina
3.2.1.125 raucaffricine glucosidase
-
 Rauvolfia serpentina