Literature summary extracted from

Wu, T.K.; Liu, Y.T.; Chiu, F.H.; Chang, C.H.
Phenylalanine 445 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences C-Ring cyclization and deprotonation reactions (2006), Org. Lett., 8, 4691-4694.

Protein Variants

EC Number	Protein Variants	Comment	Organism
5.4.99.7	F445C	produces 10% (13alphaH)-iso-malabarica-14(16)-17E,21-trien-3beta-ol, 69% lanosterol, 13% parkeol, 8% 9beta-lanosta-7,24-dien-3beta-ol from substrate (S)-2,3-epoxysqualene	Saccharomyces cerevisiae
5.4.99.7	F445D	produces 21% (13alphaH)-iso-malabarica-14(16)-17E,21-trien-3beta-ol, 63% lanosterol, 11% parkeol, 5% 9beta-lanosta-7,24-dien-3beta-ol from substrate (S)-2,3-epoxysqualene	Saccharomyces cerevisiae
5.4.99.7	F445M	produces 7% (13alphaH)-iso-malabarica-14(16)-17E,21-trien-3beta-ol, 65% lanosterol, 18% parkeol, 10% 9beta-lanosta-7,24-dien-3beta-ol from substrate (S)-2,3-epoxysqualene	Saccharomyces cerevisiae
5.4.99.7	F445N	produces 10% (13alphaH)-iso-malabarica-14(16)-17E,21-trien-3beta-ol, 63% lanosterol, 9% parkeol, 18% 9beta-lanosta-7,24-dien-3beta-ol from substrate (S)-2,3-epoxysqualene	Saccharomyces cerevisiae
5.4.99.7	F445T	produces 49% (13alphaH)-iso-malabarica-14(16)-17E,21-trien-3beta-ol, 46% lanosterol, 5% 9beta-lanosta-7,24-dien-3beta-ol from substrate (S)-2,3-epoxysqualene	Saccharomyces cerevisiae
5.4.99.7	additional information	only polar side-chain group substitutions of F445 genetically complement yeast viability and produce spatially related product diversity	Saccharomyces cerevisiae

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.4.99.7	Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5.4.99.7	(S)-2,3-epoxysqualene	-	Saccharomyces cerevisiae	lanosterol	lanosterol is the only product in wild-type	?

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Release 2023.1 (January 2023)