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Literature summary extracted from

  • Scaffidi, A.; Stubbs, K.A.; Dennis, R.J.; Taylor, E.J.; Davies, G.J.; Vocadlo, D.J.; Stick, R.V.
    A 1-acetamido derivative of 6-epi-valienamine: an inhibitor of a diverse group of beta-N-acetylglucosaminidases (2007), Org. Biomol. Chem., 5, 3013-3019.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in Escherichia coli Homo sapiens
3.2.1.52 expressed in Escherichia coli BL21 Bacteroides thetaiotaomicron

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.52 complex of Bacteroides thetaiotaomicron BtGH84 with the inhibitor, analysis at 2 A resolution, data quality and structure refinement statistics shown, unsaturated ring is distorted away from its favoured solution phase conformation indicating potential for improved inhibitor potency Bacteroides thetaiotaomicron

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 N-[(1S,2R,5R,6R)-2-amino-5,6-dihydroxy-4-(hydroxymethyl)-cyclohex-3-en-1-yl]acetamide hydrochloride 1-acetamido derivative of 6-epi-valienamine, route enabling synthesis described Bacteroides thetaiotaomicron
3.2.1.52 N-[(1S,2R,5R,6R)-2-amino-5,6-dihydroxy-4-(hydroxymethyl)-cyclohex-3-en-1-yl]acetamide hydrochloride 1-acetamido derivative of 6-epi-valienamine, route enabling synthesis described Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bacteroides thetaiotaomicron
-
 BtGH84 complex
-
3.2.1.52 Homo sapiens
-
 inhibition assay of human beta-N-acetyglucosaminidase by an analogue of 6-epi-valienamine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52
-
 Homo sapiens
3.2.1.52 gel filtration Bacteroides thetaiotaomicron

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 additional information
-
 reaction velocities for continuous based assays determined by monitoring the liberation of 4-nitrophenolate Homo sapiens
3.2.1.52 additional information
-
 reaction velocities for continuous based assays determined by monitoring the liberation of 4-nitrophenolate Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + N-acetyl-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Bacteroides thetaiotaomicron 4-nitrophenol + N-acetyl-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 beta-N-acetylglucosaminidase
-
 Homo sapiens
3.2.1.52 beta-N-acetylglucosaminidase
-
 Bacteroides thetaiotaomicron

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 37
-
 assay at Homo sapiens
3.2.1.52 37
-
 assay at Bacteroides thetaiotaomicron

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.52 0.0062
-
 N-[(1S,2R,5R,6R)-2-amino-5,6-dihydroxy-4-(hydroxymethyl)-cyclohex-3-en-1-yl]acetamide hydrochloride substrate concentration 0.5 mM, two enzyme concentrations of 0.0406 and 0.012 micrograms per microliter Homo sapiens
3.2.1.52 0.026
-
 N-[(1S,2R,5R,6R)-2-amino-5,6-dihydroxy-4-(hydroxymethyl)-cyclohex-3-en-1-yl]acetamide hydrochloride substrate concentration 0.5 mM, two enzyme concentrations of 0.04 and 0.012 micrograms per microliter Bacteroides thetaiotaomicron