EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.14.1 | additional information | strong stimulatory effects of the mcm5 group, weak positive effect of the s2 group and a negative effect of the bacterial 5-methylaminomethyl group | Kluyveromyces lactis | |
3.1.14.1 | Trimethylamine N-oxide | enhances cleavage of unmodified tRNA and anticodon stem loops by gamma-toxin-GST | Kluyveromyces lactis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.14.1 | gamma-toxin-GST expressed in Escherichia coli BL21(DE3) | Kluyveromyces lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.16 | tRNA + H2O | Escherichia coli | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.14.1 | Kluyveromyces lactis | - |
- |
- |
4.6.1.16 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.14.1 | - |
Kluyveromyces lactis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Storage Stability | Organism |
---|---|---|
3.1.14.1 | -20°C, 50% glycerol, stable for 12 months | Kluyveromyces lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.14.1 | RNA + H2O | in vitro transcribed tRNAGlu3 UUC, tRNAGIn UUG and tRNALys UUU are substrates, stable anticodon stem and the anticodon loop are the minimal requirements for cleavage, synthetic minihelix RNA corresponding to the anticodon stem loop of the natural substrate tRNAGlu3 mcm5s2UUC is cleaved at the same position as the natural substrate. In ASLGlu3 UUC, the nucleotides U34U35C36A37C38 are required for optimal gamma-toxin cleavage, whereas a purine at position 32 or a G in position 33 dramatically reduces the cleavage of the anticodon stem loop | Kluyveromyces lactis | nucleoside 3'-phosphate + ? | - |
? | |
4.6.1.16 | tRNA + H2O | - |
Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.14.1 | Gamma-toxin | - |
Kluyveromyces lactis |
3.1.14.1 | tRNA endonuclease | - |
Kluyveromyces lactis |
4.6.1.16 | Gamma-toxin | - |
Escherichia coli |
4.6.1.16 | tRNA anticodon nucleases (ACNs) | - |
Escherichia coli |