Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gao, H.; Huang, J.; Barany, F.; Cao, W.
    Switching base preferences of mismatch cleavage in endonuclease V: an improved method for scanning point mutations (2007), Nucleic Acids Res., 35, e2/1-e2/6.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.7 additional information high pH, metal cofactor Mn2+, using excess enzyme, and/or solvents such as dimethyl sulfoxide and betaine, lead to cleavage of most mismatched DNA base pairs Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.7 G41V mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 H125A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 H214D mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 I81A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 P207A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 P209A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 P82A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 R118A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 R205K mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 R211A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 R211K mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 R88E mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 R88K mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 R88Q mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
3.1.21.7 Y80A conversion to a C-specific mismatch cleavage variant Thermotoga maritima
3.1.21.7 Y80F mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.7 Mn2+ enhanced mismatch cleavage Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.7 Thermotoga maritima Q9X2H9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.7 DNA + H2O cleavage of deaminated bases at the second phosphodiester bond 3' downstream to a lesion Thermotoga maritima hydrolysed DNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.7 endonuclease V
-
 Thermotoga maritima