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Literature summary extracted from

  • Mechold, U.; Fang, G.; Ngo, S.; Ogryzko, V.; Danchin, A.
    YtqI from Bacillus subtilis has both oligoribonuclease and pAp-phosphatase activity (2007), Nucleic Acids Res., 35, 4552-4561.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.13.3 additional information YtqI, in addition to oligoribonuclease activity, has 3'-phosphoadenosine 5'-phosphate-phosphatase activity in vitro. YtqI is able to complement both orn and cysQ mutants in Escherichia coli Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.13.3 expression of his-tagged YtqI under control of the arabinose-inducible promoter Para Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.13.3 additional information ORN mutant, shows growth defect, YtqI from Bacillus subtilis is able to complement the mutant Escherichia coli
3.1.13.3 additional information ytqI mutant, shows impairment of growth in the absence of cysteine Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.13.3 3'-phosphoadenosine 5'-phosphate inhibits YtqI-catalyzed degradation of nanoRNA. At 0.1, 0.2 or 0.5 mM, the activity of YtqI based on the conversion of 5-mer into monomers in 30 min dropps to 28, 4 and 1%, respectively Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.3 Mn2+
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.3 Bacillus subtilis
-
-
-
3.1.13.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.3
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.3 3'-phosphoadenosine 5'-phosphate + H2O
-
 Bacillus subtilis AMP + phosphate
-
 ?
3.1.13.3 RNA + H2O degrades RNA oligonucleotides with preference for 3-mers Bacillus subtilis nucleoside 5'-phosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.13.3 ORN
-
 Escherichia coli
3.1.13.3 YtqI
-
 Bacillus subtilis