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Literature summary extracted from

  • Minagawa, A.; Takaku, H.; Ishii, R.; Takagi, M.; Yokoyama, S.; Nashimoto, M.
    Identification by Mn2+ rescue of two residues essential for the proton transfer of tRNase Z catalysis (2006), Nucleic Acids Res., 34, 3811-3818.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.11 expressed in Escherichia coli, pQE-80L vector used for generation of engineered variants containing a single amino acid substitution, His-tagging Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.26.11 four-layer alpha-beta/beta-alpha sandwich fold determined Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.11 D190A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 D25A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 D52A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 F11P in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 H134A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 H222A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 H244A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 H48A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 H50A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima
3.1.26.11 H53A in vitro 3'-processing in the presence of 10mM Mg2+ or 0.2 mM Mn2+ Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.11 Co2+ 0.2 mM, no rescue of lost tRNase Z activity observed Thermotoga maritima
3.1.26.11 Cu2+ 0.2 mM, no rescue of lost tRNase Z activity observed Thermotoga maritima
3.1.26.11 Mg2+ 10 mM, reaction kinetics indicated, cleavage by each variant in the presence of Mg2+ hardly detected, binding to pre-tRNA observed, essential for activity Thermotoga maritima
3.1.26.11 Mn2+ 0.2 mM, reaction kinetics indicated, essential for activity, Mn2+ ions restore a lost Mg2+ dependent activity with two exceptions of the Asp52Ala and His222Ala substitutions Thermotoga maritima
3.1.26.11 Zn2+ 0.2 mM, no rescue of lost tRNase Z activity observed Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.11 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.11 gel filtration Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.26.11 additional information
-
 reaction kinetics for in vitro 3'-processing of pre-tRNAArg(74CCA76) and pre-tRNAArg(74GUG76) in the presence of 10mM Mg2+ or 0.2 mM Mn2+ shown Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.11 pre-tRNAArg(74CCA76) + H2O reaction kinetics for in vitro 3'-processing using engineered enzyme variants, in presence of 10 mM Mg2+ or of 0.2 mM Mn2+ Thermotoga maritima ?
-
 ?
3.1.26.11 pre-tRNAArg(74GUG76) + H2O cleavage of pre-tRNAArg(74GUG76) by engineered variants about 7 to more than 16fold less efficient than that of pre-tRNAArg(74CCA76) Thermotoga maritima ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.11 tRNase Z
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.11 60
-
 assay at Thermotoga maritima