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Literature summary extracted from

  • Le Roy, K.; Verhaest, M.; Rabijns, A.; Clerens, S.; Van Laere, A.; Van den Ende, W.
    N-glycosylation affects substrate specificity of chicory fructan 1-exohydrolase: evidence for the presence of an inulin binding cleft (2007), New Phytol., 176, 317-324.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 expressed in Pichia pastoris Cichorium intybus
3.2.1.80 expression in Pichia pastoris Cichorium intybus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.80 P294N mutant with an introduced N-glycosylation site near the inulin-binding cleft, shows highly decreased activity against higher DP inulin. The introduction of a glycosyl chain most probably blocks the cleft and prevents inulin binding and degradation Cichorium intybus
3.2.1.80 P294N shows highly decreased activity against inulin Cichorium intybus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 additional information glycosylation of 1-FEH IIa affected overall enzyme activity Cichorium intybus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Cichorium intybus
-
-
-
3.2.1.80 Cichorium intybus Q93X60 precursor
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.80 glycoprotein glycosylation of 1-FEH IIa affects overall enzyme activity Cichorium intybus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80
-
 Cichorium intybus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 1-kestose + H2O
-
 Cichorium intybus beta D-fructofuranose + sucrose
-
 ?
3.2.1.80 1-kestose + H2O
-
 Cichorium intybus beta-D-fructose + sucrose
-
 ?
3.2.1.80 fructan + H2O
-
 Cichorium intybus beta-D-fructose + ?
-
 ?
3.2.1.80 inulin + 5 H2O
-
 Cichorium intybus 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 1-FEH IIa
-
 Cichorium intybus
3.2.1.80 fructan 1-exohydrolase
-
 Cichorium intybus