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Literature summary extracted from

  • Verhaest, M.; Lammens, W.; Le Roy, K.; De Ranter, C.J.; Van Laere, A.; Rabijns, A.; Van den Ende, W.
    Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitor (2007), New Phytol., 174, 90-100.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 expressed in Escherichia coli Top10 cells Cichorium intybus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.80 hanging-drop vapour diffusion method, crystallization of complexes between 1-FEH IIa with the substrate 1-kestose and the inhibitors sucrose, fructose and 2,5-dideoxy-2,5-imino-D-mannitol Cichorium intybus
3.2.1.80 mutant proteins are crystallized by hanging drop vapour diffusion method with 2.1 M sodium potassium phosphate and 0.1 M potassium phosphate, pH 7.0 Cichorium intybus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.80 E201Q inactive Cichorium intybus
3.2.1.80 S101L inactive Cichorium intybus
3.2.1.80 S101L no inhibition by sucrose Cichorium intybus
3.2.1.80 W82L inactive Cichorium intybus
3.2.1.80 W82L no inhibition by sucrose Cichorium intybus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 2,5 dideoxy-2,5-imino-D-mannitol
-
 Cichorium intybus
3.2.1.80 D-fructose
-
 Cichorium intybus
3.2.1.80 sucrose 63% inhibition by 10 mM sucrose, wild-type enzyme. Mutant enzymes W82L and S101L are not inhibited by sucrose. W82 and S101 are important for binding sucrose as inhibitor Cichorium intybus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Cichorium intybus Q93X60 precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80
-
 Cichorium intybus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 1-kestose + H2O
-
 Cichorium intybus ?
-
 ?
3.2.1.80 1-kestose + H2O
-
 Cichorium intybus beta-D-fructose + sucrose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 1-FEH IIa
-
 Cichorium intybus
3.2.1.80 fructan 1-exohydrolase
-
 Cichorium intybus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.80 3.5
-
 sucrose
-
 Cichorium intybus