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Literature summary extracted from

  • Marques, M.R.; Buckeridge, M.S.; Braga, M.R.; Dietrich, S.M.
    Characterization of an extracellular endopolygalacturonase from the saprobe Mucor ramosissimus Samutsevitsch and its action as trigger of defensive response in tropical plants (2006), Mycopathologia, 162, 337-346.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.15 additional information besides their role in recycling organic matter, saprobiotic enzymes like endopolygalacturonase may also play an important role in the induction of defensive mechanisms in wild plants by enhancing their non-specific resistance against pathogens Mucor ramosissimus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 24000
-
 x * 24000, SDS-PAGE Mucor ramosissimus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Mucor ramosissimus
-
-
-
3.2.1.15 Mucor ramosissimus URM 3087
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 partially purified by ion exchange chromatography, gel filtration, and preparative isoelectrofocusing, up to 207fold Mucor ramosissimus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.15 mycelium
-
 Mucor ramosissimus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 542
-
 culture filtrate Mucor ramosissimus
3.2.1.15 112600
-
 207fold purified enzyme Mucor ramosissimus

Storage Stability

EC Number Storage Stability Organism
3.2.1.15 -18°C, for 180 days Mucor ramosissimus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 pectin + H2O pectin from citrus or Palicourea marcgravii, more active upon pectic substrates with a low degree of methyl esterification. The enzyme itself and the products of its action on the pectic fraction of Palicourea marcgravii elicite the production of defensive compounds in the leaves of the plant Mucor ramosissimus oligogalacturonides
-
 ?
3.2.1.15 pectin + H2O pectin from citrus or Palicourea marcgravii, more active upon pectic substrates with a low degree of methyl esterification. The enzyme itself and the products of its action on the pectic fraction of Palicourea marcgravii elicite the production of defensive compounds in the leaves of the plant Mucor ramosissimus URM 3087 oligogalacturonides
-
 ?
3.2.1.15 polygalacturonic acid + H2O
-
 Mucor ramosissimus ?
-
 ?
3.2.1.15 polygalacturonic acid + H2O
-
 Mucor ramosissimus URM 3087 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 24000, SDS-PAGE Mucor ramosissimus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endopolygalacturonase
-
 Mucor ramosissimus