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Literature summary extracted from

  • Todo, T.; Ryo, H.; Borden, A.; Lawrence, C.; Sakaguchi, K.; Hirata, H.; Nomura, T.
    Non-mutagenic repair of (6-4)photoproducts by (6-4)photolyase purified from Drosophila melanogaster (1997), Mutat. Res., 385, 83-93.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.13 62000
-
 SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.13 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.13 purified to near homogeneity from Drosophila embryonic cells by using NH4SO4 precipitation, SP sepharose column, UV DNA affinity beads, heparin sepharose column and Mono S column Drosophila melanogaster

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.99.13 (6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases.This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases Drosophila melanogaster
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.13 C(6-4)C photoproduct (in DNA)
-
 Drosophila melanogaster 2 cytosine residues (in DNA)
-
 ?
4.1.99.13 T(6-4)C photoproduct (in DNA)
-
 Drosophila melanogaster 2 thymidine + cytosine (in DNA)
-
 ?
4.1.99.13 T(6-4)T photoproduct (in DNA)
-
 Drosophila melanogaster 2 thymidine resdiues (in DNA)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.13 (6-4) photolyase
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.13 FAD
-
 Drosophila melanogaster