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Literature summary extracted from

  • Hammarton, T.C.; Kramer, S.; Tetley, L.; Boshart, M.; Mottram, J.C.
    Trypanosoma brucei Polo-like kinase is essential for basal body duplication, kDNA segregation and cytokinesis (2007), Mol. Microbiol., 65, 1229-1248.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.11.21 additional information role for PLK kinase activity in basal body and kinetoplast migration, PLK is essential for cytokinesis furrow ingression in bloodstream-form Trypanosoma brucei Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.21 overexpression of TY1-epitope-tagged PLK and a kinase-dead variant, results in the accumulation of cells that have divided their nucleus but not their kinetoplast (2N1K cells), kinetoplast is mainly found at the posterior end of the cell Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.21 N169A abrogates kinase activity, after PLK depletion the single kinetoplast is predominantly located between the two divided nuclei Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.21 RNA downregulates PLK, in procyclic forms inhibition of kinetoplast division, in bloodstream form also delayed kinetoplast division, which is further observed to inhibit furrow ingression during cytokinesis Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.21 cytoplasm
-
 Trypanosoma brucei 5737
-
2.7.11.21 additional information no localization to the nucleus, kinetoplast, basal bodies or to the flagellum Trypanosoma brucei
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.21 Trypanosoma brucei
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.21 bloodstream form
-
 Trypanosoma brucei
-
2.7.11.21 procyclic form
-
 Trypanosoma brucei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.21 ATP + alpha-casein
-
 Trypanosoma brucei ADP + phosphorylated alpha-casein
-
 ?
2.7.11.21 ATP + beta-casein
-
 Trypanosoma brucei ADP + phosphorylated beta-casein
-
 ?
2.7.11.21 additional information no activity against myelin basic protein or histone H1 Trypanosoma brucei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.21 PLK
-
 Trypanosoma brucei
2.7.11.21 polo-like kinase
-
 Trypanosoma brucei