BRENDA - Enzyme Database

Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis

Gould, T.A.; van de Langemheen, H.; Munoz-Elias, E.J.; McKinney, J.D.; Sacchettini, J.C.; Mol. Microbiol. 61, 940-947 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.1.3.1
vapour diffusion with 0.1 M Tris-HCl, 0.2 M sodium acetate, 20-30% (w/v) PEG 4000, pH 8.0
Mycobacterium tuberculosis
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
C191A
unable to form the closed conformation crystal form
Mycobacterium tuberculosis
4.1.3.1
C195A
reduced activity
Mycobacterium tuberculosis
4.1.3.1
C195S
reduced activity
Mycobacterium tuberculosis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.188
-
isocitrate
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2
Mycobacterium tuberculosis
4.1.3.1
0.718
-
(2R,3S:2S,3R)-2-methyl-isocitrate
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2
Mycobacterium tuberculosis
4.1.3.1
1.14
-
isocitrate
ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2
Mycobacterium tuberculosis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
required
Mycobacterium tuberculosis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Mycobacterium tuberculosis
P9WKK7
-
-
4.1.3.1
Mycobacterium tuberculosis H37Rv
P9WKK7
-
-
4.1.3.30
Mycobacterium tuberculosis
-
-
-
4.2.1.79
Mycobacterium tuberculosis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
HiTrap Q FF ion exchange column chromatography, Superdex S-200 gel filtration, and HiTrap Q HP ion exchange column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S:2S,3R)-2-methyl-isocitrate
-
681995
Mycobacterium tuberculosis
succinate + pyruvate
-
-
-
?
4.1.3.1
isocitrate
-
681995
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
?
4.1.3.30
(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate
-
681995
Mycobacterium tuberculosis
Pyruvate + succinate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
x-ray crystallography
Mycobacterium tuberculosis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.3.1
1.25
-
(2R,3S:2S,3R)-2-methyl-isocitrate
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2
Mycobacterium tuberculosis
4.1.3.1
1.38
-
isocitrate
ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2
Mycobacterium tuberculosis
4.1.3.1
5.24
-
isocitrate
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.1.3.1
vapour diffusion with 0.1 M Tris-HCl, 0.2 M sodium acetate, 20-30% (w/v) PEG 4000, pH 8.0
Mycobacterium tuberculosis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
C191A
unable to form the closed conformation crystal form
Mycobacterium tuberculosis
4.1.3.1
C195A
reduced activity
Mycobacterium tuberculosis
4.1.3.1
C195S
reduced activity
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.188
-
isocitrate
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2
Mycobacterium tuberculosis
4.1.3.1
0.718
-
(2R,3S:2S,3R)-2-methyl-isocitrate
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2
Mycobacterium tuberculosis
4.1.3.1
1.14
-
isocitrate
ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2
Mycobacterium tuberculosis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
required
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
HiTrap Q FF ion exchange column chromatography, Superdex S-200 gel filtration, and HiTrap Q HP ion exchange column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S:2S,3R)-2-methyl-isocitrate
-
681995
Mycobacterium tuberculosis
succinate + pyruvate
-
-
-
?
4.1.3.1
isocitrate
-
681995
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
?
4.1.3.30
(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate
-
681995
Mycobacterium tuberculosis
Pyruvate + succinate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
x-ray crystallography
Mycobacterium tuberculosis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.3.1
1.25
-
(2R,3S:2S,3R)-2-methyl-isocitrate
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2
Mycobacterium tuberculosis
4.1.3.1
1.38
-
isocitrate
ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2
Mycobacterium tuberculosis
4.1.3.1
5.24
-
isocitrate
ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2
Mycobacterium tuberculosis