EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.13.3 | additional information | hybrid histidine protein kinase in which four signalling domains are required for full activity | Myxococcus xanthus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.13.3 | wild-type and mutants introduced into deltarodK strain SA1708, His6-tagged wild-type and mutants expressed in Escherichia coli | Myxococcus xanthus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.13.3 | D657N | substitution in the first receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, rare sporulation outside fruiting bodies, but normal fruiting body morphology on CF agar, loose aggregates of fruiting bodies in submerged culutre | Myxococcus xanthus |
2.7.13.3 | D657N/D782N | delayed timing of aggregation, frequent sporulation outside fruiting bodies, and loose mounds of fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, no autophosphorylation | Myxococcus xanthus |
2.7.13.3 | D657N/D782N/D909N | delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates | Myxococcus xanthus |
2.7.13.3 | D657N/D909N | delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular and small fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates | Myxococcus xanthus |
2.7.13.3 | D782N | substitution in the second receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular and small fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre | Myxococcus xanthus |
2.7.13.3 | D782N/D909N | delayed timing of aggregation, frequent sporulation outside fruiting bodies, and loose mounds of fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates | Myxococcus xanthus |
2.7.13.3 | D909N | substitution in the third receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, but normal fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre | Myxococcus xanthus |
2.7.13.3 | H360A | is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre | Myxococcus xanthus |
2.7.13.3 | additional information | delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre | Myxococcus xanthus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.13.3 | 0.1 | - |
ATP | mutant D657N/D782N/D909N | Myxococcus xanthus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.13.3 | Myxococcus xanthus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.13.3 | - |
Myxococcus xanthus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.13.3 | ATP + protein L-histidine | - |
Myxococcus xanthus | ADP + protein N-phospho-L-histidine | - |
? | |
2.7.13.3 | additional information | no autophosphorylation of wild-type, is caused by the rate of dephosphorylation being higher than the rate of autophosphorylation | Myxococcus xanthus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.13.3 | histidine protein kinase | - |
Myxococcus xanthus |
2.7.13.3 | RodK | - |
Myxococcus xanthus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.13.3 | 0.000167 | - |
ATP | mutant D657N/D782N/D909N | Myxococcus xanthus |