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Literature summary extracted from

  • Burden, R.E.; Snoddy, P.; Buick, R.J.; Johnston, J.A.; Walker, B.; Scott, C.J.
    Recombinant cathepsin S propeptide attenuates cell invasion by inhibition of cathepsin L-like proteases in tumor microenvironment (2008), Mol. Cancer Ther., 7, 538-547.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.27 the cathepsin S propeptide is expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.27 cathepsin S propeptide 0.0002 mM, blocks gelatinase, collagenase and elastinase activities of mature cathepsin S Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.27 16000
-
 His-tagged recombinant propeptide, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.27 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.27 Ni2+ HiTrap column chromatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.22.27 complete removal of urea phosphate buffer starting from 8 M Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.27 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.27 Collagen + H2O
-
 Homo sapiens ?
-
 ?
3.4.22.27 Elastin + H2O
-
 Homo sapiens ?
-
 ?
3.4.22.27 Gelatin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.27 CatS mature enzyme Homo sapiens
3.4.22.27 CatSPP cathepsin S propeptide Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.27 0.0000025
-
 cathepsin S propeptide using benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin as a substrate Homo sapiens