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Literature summary extracted from

  • Mabrouk, S.B.; Messaoud, E.B.; Ayadi, D.; Jemli, S.; Roy, A.; Mezghani, M.; Bejar, S.
    Cloning and sequencing of an original gene encoding a maltogenic amylase from Bacillus sp. US149 strain and characterization of the recombinant activity (2008), Mol. Biotechnol., 38, 211-219.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133 expressed in Escherichia coli DH5alpha using expression vectors pBMS1-4, open reading frame of 1749 bp encoding a protein of 582 residues, low sequence similarity of 60% to maltogenic amylase of Thermus sp., four known conserved regions at position 241 to 246, 324 to 331, 355 to 360, and 418 to 423 identified Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.133 intracellular recombinant enzyme MAUS149 Bacillus sp. (in: Bacteria) 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.133 67500
-
 each of the two subunits of the dimer Bacillus sp. (in: Bacteria)
3.2.1.133 135000
-
 SDS-PAGE, gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.133 starch + H2O Bacillus sp. (in: Bacteria) maltogenic amylase from Bacillus sp. alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O Bacillus sp. (in: Bacteria) US149 maltogenic amylase from Bacillus sp. alpha-maltose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Bacillus sp. (in: Bacteria) A7DWA8 ma gene; US149
-
3.2.1.133 Bacillus sp. (in: Bacteria) US149 A7DWA8 ma gene; US149
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133 recombinant protein, from cell crude extracts with a yield of 23% Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.133 additional information
-
 kinetic mechanism for recombinant enzyme MAUS149 shown, determined by measuring the amount of reducing sugars released during incubation with starch using the dinitrosalicylic acid method, substrate concentrations ranging from 1 to 7.5 g/ml for starch and from 0.5 to 4 g/l for cyclodextrin and pullulan Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 beta-cyclodextrin + H2O substrate determination for recombinant enzyme MAUS149 Bacillus sp. (in: Bacteria) alpha-maltose + glucose
-
 ?
3.2.1.133 beta-cyclodextrin + H2O substrate determination for recombinant enzyme MAUS149 Bacillus sp. (in: Bacteria) US149 alpha-maltose + glucose
-
 ?
3.2.1.133 pullulan + H2O substrate determination for recombinant enzyme MAUS149 Bacillus sp. (in: Bacteria) alpha-maltose + ?
-
 ?
3.2.1.133 pullulan + H2O substrate determination for recombinant enzyme MAUS149 Bacillus sp. (in: Bacteria) US149 alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O maltogenic amylase from Bacillus sp. Bacillus sp. (in: Bacteria) alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O substrate determination for recombinant enzyme MAUS149 Bacillus sp. (in: Bacteria) alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O maltogenic amylase from Bacillus sp. Bacillus sp. (in: Bacteria) US149 alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O substrate determination for recombinant enzyme MAUS149 Bacillus sp. (in: Bacteria) US149 alpha-maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.133 dimer 2 * 67500, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 glucan 1,4-alpha-maltohydrolase
-
 Bacillus sp. (in: Bacteria)
3.2.1.133 maltogenic amylase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 40
-
 maximal activity, effect of temperature on activity investigated in a 50 mM sodium phosphate buffer at pH 6.5 using different temperatures ranging from 10°C to 60°C Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.133 10 60 kinetics shown, more than 50% of activity retained at the temperature range of 10–50°C, rapid decrease in activity above 60°C Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.133 additional information
-
 kinetics shown, stable at 45°C for 90 min, half-life duration of 18 min at 55°C Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.133 6.5
-
 maximal activity, effect of pH on activity studied at 40°C using 50 mM buffers with different pH values ranging from 3.5 to 10 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.133 5 9.5 more than 80% of enzyme activity retained at pH ranges from 5 to 7, recombinant maltogenic amylase stable at pH ranging from 5 to 9.5 and mainly between 6 and 8 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.133 additional information
-
 determined in different buffers ranging from pH 5 to pH 10 for 12 h at 4°C followed by activity assay Bacillus sp. (in: Bacteria)