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Literature summary extracted from

  • Diaz, J.C.; Cordova, J.; Baratti, J.; Carriere, F.; Abousalham, A.
    Effect of nonionic surfactants on Rhizopus homothallicus lipase activity: a comparative kinetic study (2007), Mol. Biotechnol., 35, 205-214.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 taurodeoxycholate 1.92fold activation, pH 7.5, 25°C Rhizopus homothallicus
3.1.1.3 Triton X-100 interacts with SSF and SmF lipases increasing their activity 1.7fold, pH 7.5, 25°C Rhizopus homothallicus
3.1.1.3 Tween 80 1.25fold activation, pH 7.5, 25°C Rhizopus homothallicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 diethyl 4-nitrophenyl phosphate i.e. E600, 80% and 60% inhibition of SSF lipase and SmF lipase, respectively, pH 7.5, 25°C Rhizopus homothallicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 29500
-
 SSF and SmF lipases, gel filtration Rhizopus homothallicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Rhizopus homothallicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 SSF and SmF lipases by hydrophobic interaction chromatography and gel filtration Rhizopus homothallicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 additional information solid and submerged state fermentation methods Rhizopus homothallicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 8600
-
 SmF lipase Rhizopus homothallicus
3.1.1.3 10700
-
 SSF lipase Rhizopus homothallicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 trioctanoin + H2O
-
 Rhizopus homothallicus dioctanoin + octanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Rhizopus homothallicus
3.1.1.3 SmF lipase
-
 Rhizopus homothallicus
3.1.1.3 SSF lipase
-
 Rhizopus homothallicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Rhizopus homothallicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 assay at Rhizopus homothallicus