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Literature summary extracted from

  • Salazar, O.; Basso, C.; Barba, P.; Orellana, C.; Asenjo, J.A.
    Improvement of the lytic properties of a beta-1,3-glucanase by directed evolution (2006), Mol. Biotechnol., 33, 211-220.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expressed in Escherichia coli BL21(DE3) cells Cellulosimicrobium cellulans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 F160L increased activity Cellulosimicrobium cellulans
3.2.1.39 G145D increased activity Cellulosimicrobium cellulans
3.2.1.39 T149A/A344V increased activity Cellulosimicrobium cellulans
3.2.1.39 T149A/G145D/A344V increased activity Cellulosimicrobium cellulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 0.35
-
 Laminarin mutant enzyme F160L, at 37°C Cellulosimicrobium cellulans
3.2.1.39 0.41
-
 Laminarin wild type enzyme, at 37°C Cellulosimicrobium cellulans
3.2.1.39 0.44
-
 Laminarin mutant enzyme T149A/A344V, at 37°C Cellulosimicrobium cellulans
3.2.1.39 0.46
-
 Laminarin mutant enzyme G145D, at 37°C Cellulosimicrobium cellulans
3.2.1.39 0.48
-
 Laminarin mutant enzyme T149A/G145D/A344V, at 37°C Cellulosimicrobium cellulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 40000
-
 SDS-PAGE Cellulosimicrobium cellulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Cellulosimicrobium cellulans O68641 formerly Oerskovia xanthineolytica, strain LLG109
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 nickel metal affinity chromatography Cellulosimicrobium cellulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 49.1
-
 wild type enzyme, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans
3.2.1.39 70.3
-
 mutant enzyme F160L, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans
3.2.1.39 80.6
-
 mutant enzyme T149A/A344V, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans
3.2.1.39 96.2
-
 mutant enzyme G145D, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans
3.2.1.39 162.7
-
 mutant enzyme T149A/G145D/A344V, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 carboxymethyl-curdlan + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Cellulosimicrobium cellulans D-glucose + ?
-
 ?
3.2.1.39 lichenan + H2O
-
 Cellulosimicrobium cellulans D-glucose + ?
-
 ?
3.2.1.39 zymosan A + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 beta-1,3-glucanase
-
 Cellulosimicrobium cellulans
3.2.1.39 BglII
-
 Cellulosimicrobium cellulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.39 2.1
-
 Laminarin mutant enzyme T149A/A344V, at 37°C Cellulosimicrobium cellulans
3.2.1.39 35.9
-
 Laminarin wild type enzyme, at 37°C Cellulosimicrobium cellulans
3.2.1.39 51.5
-
 Laminarin mutant enzyme F160L, at 37°C Cellulosimicrobium cellulans
3.2.1.39 62.4
-
 Laminarin mutant enzyme G145D, at 37°C Cellulosimicrobium cellulans
3.2.1.39 72.8
-
 Laminarin mutant enzyme T149A/A344V, at 37°C Cellulosimicrobium cellulans
3.2.1.39 126.6
-
 Laminarin mutant enzyme T149A/G145D/A344V, at 37°C Cellulosimicrobium cellulans
3.2.1.39 138
-
 Laminarin mutant enzyme T149A/G145D/A344V, at 37°C Cellulosimicrobium cellulans