EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.53 | of MYPT1 in yeast AH109 strain for yeast two hybrid screening, MLCP holoenzyme in Sf9 cells by coinfection of rat His MYPT1, Flag MYPT1, rat PP1delta, and rat M21 expressing viruses, transfection of COS7 or NIH3T3 cells | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.53 | additional information | deletion of the C-terminal residues of MYPT1 at codon 39, 172, 297 and 517 by site directed mutagenesis | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.53 | 14-3-3beta protein | 70% inhibition in the presence of 0.5 mM 14-3-3beta, dissociates MLCP from myosin II | Rattus norvegicus | |
3.1.3.53 | additional information | MLCP holoenzyme is inhibited by binding of 14-3-3beta protein to MYPT1, 14-3-3beta protein diminishes the binding between MYPT1 and myosin II, and abolishes MYPT1 localization at stress fibers | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.53 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.53 | Flag MYPT1 using anti-Flag affinity chromatography, His tagged proteins by Ni-NTA chromatography | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.53 | myosin light-chain phosphate + H2O | muscle | Rattus norvegicus | myosin light-chain + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.53 | More | MLCP is a heterotrimer composed of the catalytic subunit belonging to protein phosphatase 1c PPC1, the myosin phosphatase targeting subunit MYPT1, and M21 | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.53 | mLC phosphatase | - |
Rattus norvegicus |
3.1.3.53 | MLCP | - |
Rattus norvegicus |
3.1.3.53 | myosin light chain phosphatase | - |
Rattus norvegicus |
3.1.3.53 | MYPT1 | - |
Rattus norvegicus |