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Literature summary extracted from

  • Colasante, C.; Robles, A.; Li, C.H.; Schwede, A.; Benz, C.; Voncken, F.; Guilbride, D.L.; Clayton, C.
    Regulated expression of glycosomal phosphoglycerate kinase in Trypanosoma brucei (2007), Mol. Biochem. Parasitol., 151, 193-204.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.3 additional information in bloodstream forms, after XRNA depletion, abundance of PGKB mRNA increases to about one-third of the PGKC mRNA abundance, with a corresponding decrease in the apparent degradation rate. The degradation of PGKC mRNA is not affected. In procyclic forms, PGKB mRNA is unaffected by XRNA depletion, PGKC mRNA is undetectable, but after XRNA depletion, PGKC mRNA is detectable Trypanosoma brucei

Application

EC Number Application Comment Organism
2.7.2.3 additional information PGKB mRNA and PGKB protein are much more abundant in the procyclic life-cycle stage than in bloodstream forms, whereas PGKC mRNA and PGKC protein are specific to bloodstream forms. PGKC 3'-UTR exerts strong regulatory effects, they are exerted at the levels of both mRNA stability and translation. PGKC3'-UTR contains some elements which promote active degradation in procyclic forms, but it also is responsible for an active stabilisation process in bloodstream forms Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 actinomycin D blocks transcription Trypanosoma brucei
2.7.2.3 additional information addition of sinefungin prior to actinomycin D is the best protocol for complete and rapid inhibition of mRNA synthesis, thus in bloodstream forms, inhibition of both trans splicing and transcription results in immediate exponential decay of PGKC mRNA with a half-life of 46 min. Inhibition of transcription alone gives non-exponential kinetics and inhibition of splicing alone results in a longer apparent half-life Trypanosoma brucei
2.7.2.3 sinefungin blocks splicing Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.3 cytosol PGKB Trypanosoma brucei 5829
-
2.7.2.3 glycosome
-
Trypanosoma brucei 20015
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2.7.2.3 glycosome PGKC Trypanosoma brucei 20015
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.3 additional information Trypanosoma brucei PGKC 3'-UTR exerts strong regulatory effects in Trypanosoma brucei. The regulatory effects are exerted at the levels of both mRNA stability and translation ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Trypanosoma brucei
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 bloodstream form PGKB and PGKC genes-encoding phosphoglycerate kinase are co-transcribed as part of a polycistronic RNA. PGKC mRNA and glycosomal PGKC protein are specific to bloodstream forms Trypanosoma brucei
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 additional information PGKC 3'-UTR exerts strong regulatory effects in Trypanosoma brucei. The regulatory effects are exerted at the levels of both mRNA stability and translation Trypanosoma brucei ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.3 PGKB
-
Trypanosoma brucei
2.7.2.3 PGKC
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Trypanosoma brucei