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Literature summary extracted from

  • van der Kaaij, R.M.; Janecek, S.; van der Maarel, M.J.; Dijkhuizen, L.
    Phylogenetic and biochemical characterization of a novel cluster of intracellular fungal alpha-amylase enzymes (2007), Microbiology, 153, 4003-4015.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 AmyD Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information Km for potato starch in the hydrolysis reaction is between 0.02 and 0.05% starch (w/v), AmyD Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 intracellular AmyD Aspergillus niger 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 AmyD Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 2.2
-
 AmyD Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O 66% of the activity with potato starch, AmyD Aspergillus niger ?
-
 ?
3.2.1.1 amylose + H2O 101% of the activity with potato starch, AmyD Aspergillus niger ?
-
 ?
3.2.1.1 glycogen + H2O 3.6% of the activity with potato starch, AmyD Aspergillus niger ?
-
 ?
3.2.1.1 starch + H2O AmyD produces mainly maltotriose Aspergillus niger maltotriose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 AmyD
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 35
-
 10 min, stable Aspergillus niger
3.2.1.1 40
-
 10 min, about 15% loss of activity Aspergillus niger
3.2.1.1 45
-
 10 min, about 80% loss of activity Aspergillus niger
3.2.1.1 50
-
 10 min, complete loss of activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5.5 6.5 AmyD Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 5 8 pH 5.0: about 80% of maximal activity, pH 8.0: about 50% of maximal activity, AmyD Aspergillus niger