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Literature summary extracted from

  • Kolberg, J.; Aase, A.; Bergmann, S.; Herstad, T.K.; Rodal, G.; Frank, R.; Rohde, M.; Hammerschmidt, S.
    Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface (2006), Microbiology, 152, 1307-1317.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expressed in Escherichia coli host strain M15-pREP4, His-tagged protein Streptococcus pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 cytoplasm low amounts of surface-exposed enolase, determined on viable pneumococci, monoclonal and polyconal antibodies used Streptococcus pneumoniae 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Streptococcus pneumoniae
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Streptococcus pneumoniae Q97QS2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 recombinant protein, for epitope mapping and identification of plasminogen-binding motifs Streptococcus pneumoniae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 bacteroid
-
 Streptococcus pneumoniae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 additional information
-
 purified recombinant enolase has plasminogen binding activity, analyzed by flow cytometry Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Streptococcus pneumoniae phosphoenolpyruvate + H2O
-
 r
4.2.1.11 2-phospho-D-glycerate plasminogen-binding activity besides metabolic function Streptococcus pneumoniae phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 More epitope for plasminogen-binding localized in a surface-exposed loop in each of the monomers of the octameric enolase Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Streptococcus pneumoniae