BRENDA - Enzyme Database

Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface

Kolberg, J.; Aase, A.; Bergmann, S.; Herstad, T.K.; Rodal, G.; Frank, R.; Rohde, M.; Hammerschmidt, S.; Microbiology 152, 1307-1317 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli host strain M15-pREP4, His-tagged protein
Streptococcus pneumoniae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytoplasm
low amounts of surface-exposed enolase, determined on viable pneumococci, monoclonal and polyconal antibodies used
Streptococcus pneumoniae
5737
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Streptococcus pneumoniae
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Streptococcus pneumoniae
Q97QS2
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
recombinant protein, for epitope mapping and identification of plasminogen-binding motifs
Streptococcus pneumoniae
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
bacteroid
-
Streptococcus pneumoniae
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
purified recombinant enolase has plasminogen binding activity, analyzed by flow cytometry
Streptococcus pneumoniae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
681786
Streptococcus pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
plasminogen-binding activity besides metabolic function
681786
Streptococcus pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
More
epitope for plasminogen-binding localized in a surface-exposed loop in each of the monomers of the octameric enolase
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli host strain M15-pREP4, His-tagged protein
Streptococcus pneumoniae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytoplasm
low amounts of surface-exposed enolase, determined on viable pneumococci, monoclonal and polyconal antibodies used
Streptococcus pneumoniae
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Streptococcus pneumoniae
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
recombinant protein, for epitope mapping and identification of plasminogen-binding motifs
Streptococcus pneumoniae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
bacteroid
-
Streptococcus pneumoniae
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
purified recombinant enolase has plasminogen binding activity, analyzed by flow cytometry
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
681786
Streptococcus pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
plasminogen-binding activity besides metabolic function
681786
Streptococcus pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
More
epitope for plasminogen-binding localized in a surface-exposed loop in each of the monomers of the octameric enolase
Streptococcus pneumoniae