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Literature summary extracted from

  • Sehgal, A.C.; Callen, W.; Mathur, E.J.; Short, J.M.; Kelly, R.M.
    Carboxylesterase from Sulfolobus solfataricus P1. (2001), Methods Enzymol., 330, 461-471.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.45
-
 4-yethylumbelliferyl acetate 80°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 33000
-
 x * 33000, calculated from sequence Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Saccharolobus solfataricus
-
 fragment
-
3.1.1.1 Saccharolobus solfataricus P1
-
 fragment
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 recombinant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-methylumbelliferyl acetate + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + acetate
-
 ?
3.1.1.1 4-methylumbelliferyl acetate + H2O
-
 Saccharolobus solfataricus P1 4-methylumbelliferone + acetate
-
 ?
3.1.1.1 4-methylumbelliferyl butyrate + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + butyrate
-
 ?
3.1.1.1 4-methylumbelliferyl butyrate + H2O
-
 Saccharolobus solfataricus P1 4-methylumbelliferone + butyrate
-
 ?
3.1.1.1 p-nitrophenyl caproate + H2O
-
 Saccharolobus solfataricus p-nitrophenol + caproate
-
 ?
3.1.1.1 p-nitrophenyl caproate + H2O
-
 Saccharolobus solfataricus P1 p-nitrophenol + caproate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 33000, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 Sso P1 carboxylesterase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 97
-
 melting temperature Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 1000
-
 4-yethylumbelliferyl acetate 80°C Saccharolobus solfataricus
3.1.1.1 1000
-
 4-yethylumbelliferyl acetate 80°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7.7
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 5 8.5 pH 5.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity, substrate: 4-methylumbelliferyl butyrate Saccharolobus solfataricus