BRENDA - Enzyme Database

Enzymatic analysis of lipid phosphate phosphatases

McDermott, M.I.; Sigal, Y.J.; Crump, J.S.; Morris, A.J.; Methods 39, 169-179 (2006)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.1.3.4
analysis
radiolabeled and fluorescent lipid substrates for the detection, quantitation and analysis of the enzymatic activities of the LPPs measured using intact or broken cell preparations as the source of enzyme
Homo sapiens
3.1.3.4
analysis
radiolabeled and fluorescent lipid substrates for the detection, quantitation and analysis of the enzymatic activities of the LPPs measured using intact or broken cell preparations as the source of enzyme
Mus musculus
3.1.3.4
analysis
radiolabeled and fluorescent lipid substrates for the detection, quantitation and analysis of the enzymatic activities of the LPPs measured using intact or broken cell preparations as the source of enzyme
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
transient and stable overexpression in HEK-293 cells, baculovirus vectors for recombinant expression of LPPs in cultured Sf9 cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
additional information
insensitive to inhibition by the alkylating agent N-ethyl maleimide
Homo sapiens
3.1.3.4
additional information
insensitive to inhibition by the alkylating agent N-ethyl maleimide
Rattus norvegicus
3.1.3.4
propranolol
moderately effective inhibitor of LPP activity
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
membrane
-
Homo sapiens
16020
-
3.1.3.4
membrane
-
Rattus norvegicus
16020
-
3.1.3.4
membrane
-
Mus musculus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
independent of divalent metal ions
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
3.1.3.4
Mus musculus
-
-
-
3.1.3.4
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
HEK-293 cell
-
Homo sapiens
-
3.1.3.4
additional information
Swiss 3T3 cell
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide 1-phosphate + H2O
-
681760
Homo sapiens
ceramide + phosphate
-
-
-
?
3.1.3.4
ceramide 1-phosphate + H2O
-
681760
Rattus norvegicus
ceramide + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
-
681760
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
-
681760
Rattus norvegicus
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
681760
Homo sapiens
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
681760
Rattus norvegicus
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
681760
Homo sapiens
sphingosine + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
681760
Rattus norvegicus
sphingosine + phosphate
-
-
-
?
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.3.4
analysis
radiolabeled and fluorescent lipid substrates for the detection, quantitation and analysis of the enzymatic activities of the LPPs measured using intact or broken cell preparations as the source of enzyme
Homo sapiens
3.1.3.4
analysis
radiolabeled and fluorescent lipid substrates for the detection, quantitation and analysis of the enzymatic activities of the LPPs measured using intact or broken cell preparations as the source of enzyme
Mus musculus
3.1.3.4
analysis
radiolabeled and fluorescent lipid substrates for the detection, quantitation and analysis of the enzymatic activities of the LPPs measured using intact or broken cell preparations as the source of enzyme
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
transient and stable overexpression in HEK-293 cells, baculovirus vectors for recombinant expression of LPPs in cultured Sf9 cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
additional information
insensitive to inhibition by the alkylating agent N-ethyl maleimide
Homo sapiens
3.1.3.4
additional information
insensitive to inhibition by the alkylating agent N-ethyl maleimide
Rattus norvegicus
3.1.3.4
propranolol
moderately effective inhibitor of LPP activity
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
membrane
-
Homo sapiens
16020
-
3.1.3.4
membrane
-
Rattus norvegicus
16020
-
3.1.3.4
membrane
-
Mus musculus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
independent of divalent metal ions
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
HEK-293 cell
-
Homo sapiens
-
3.1.3.4
additional information
Swiss 3T3 cell
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide 1-phosphate + H2O
-
681760
Homo sapiens
ceramide + phosphate
-
-
-
?
3.1.3.4
ceramide 1-phosphate + H2O
-
681760
Rattus norvegicus
ceramide + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
-
681760
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
-
681760
Rattus norvegicus
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
681760
Homo sapiens
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
681760
Rattus norvegicus
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
681760
Homo sapiens
sphingosine + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
681760
Rattus norvegicus
sphingosine + phosphate
-
-
-
?