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Literature summary extracted from

  • Yun, C.Y.; Liu, S.; Lim, S.F.; Wang, T.; Chung, B.Y.; Jiat Teo, J.; Chuan, K.H.; Soon, A.S.; Goh, K.S.; Song, Z.
    Specific inhibition of caspase-8 and -9 in CHO cells enhances cell viability in batch and fed-batch cultures (2007), Metab. Eng., 9, 406-418.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.61 expression in Escherichia coli Cricetulus griseus
3.4.22.62 expression in Escherichia coli Cricetulus griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.61 acetyl-Asp-Glu-Val-Asp-al
-
 Cricetulus griseus
3.4.22.61 acetyl-Ile-Glu-Thr-Asp-al
-
 Cricetulus griseus
3.4.22.61 acetyl-Leu-Glu-His-Asp-al
-
 Cricetulus griseus
3.4.22.61 acetyl-Val-Asp-Val-Ala-Asp-al 0.01 mM, 80% inhibition Cricetulus griseus
3.4.22.61 benzyloxycarbonyl-Ile-Glu-Thr-Asp-fluoromethylketone
-
 Cricetulus griseus
3.4.22.61 benzyloxycarbonyl-Leu-Glu-His-Asp-fluoromethylketone
-
 Cricetulus griseus
3.4.22.61 benzyloxycarbonyl-Val-Asp-Val-Ala-Asp-fluoromethylketone
-
 Cricetulus griseus
3.4.22.61 additional information inhibition of caspase-8 enhances the viability of the CHO cells in both batch and fed-batch suspension cultures Cricetulus griseus
3.4.22.62 benzyloxycarbonyl-Ile-Glu-Thr-Asp-fluoromethylketone
-
 Cricetulus griseus
3.4.22.62 benzyloxycarbonyl-Leu-Glu-His-Asp-fluoromethylketone
-
 Cricetulus griseus
3.4.22.62 benzyloxycarbonyl-Val-Asp-Val-Ala-Asp-fluoromethylketone
-
 Cricetulus griseus
3.4.22.62 additional information inhibition of caspase-9 enhances the viability of the CHO cells in both batch and fed-batch suspension cultures Cricetulus griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.61 additional information Cricetulus griseus caspase-8 is possibly involved in the apoptotic cell death in batch and fed-batch cultures of CHO cells ?
-
 ?
3.4.22.62 additional information Cricetulus griseus caspase-9 is possibly involved in the apoptotic cell death in batch and fed-batch cultures of CHO cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.61 Cricetulus griseus
-
-
-
3.4.22.62 Cricetulus griseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.61 CHO cell inhibition of caspase-8 enhances the viability of the CHO cells in both batch and fed-batch suspension cultures. Caspase-8 is possible involved in the apoptotic cell death in batch and fed-batch cultures of CHO cells Cricetulus griseus
-
3.4.22.62 CHO cell inhibition of caspase-9 enhances the viability of the CHO cells in both batch and fed-batch suspension cultures. Caspase-9 is possible involved in the apoptotic cell death in batch and fed-batch cultures of CHO cells Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.61 acetyl-DEVD-p-nitroanilide + H2O 49% of the activity with acetyl-IETD-p-nitroanilide Cricetulus griseus acetyl-DEVD + p-nitroaniline
-
 ?
3.4.22.61 acetyl-IETD-p-nitroanilide + H2O
-
 Cricetulus griseus acetyl-IETD + p-nitroaniline
-
 ?
3.4.22.61 acetyl-LEHD-p-nitroanilide + H2O 142% of the activity with acetyl-IETD-p-nitroanilide Cricetulus griseus acetyl-LEHD + p-nitroaniline
-
 ?
3.4.22.61 acetyl-VDVAD-p-nitroanilide + H2O 36% of the activity with acetyl-IETD-p-nitroanilide Cricetulus griseus acetyl-VDVAD + p-nitroaniline
-
 ?
3.4.22.61 acetyl-VEID-p-nitroanilide + H2O 76% of the activity with acetyl-IETD-p-nitroanilide Cricetulus griseus acetyl-VEID + p-nitroaniline
-
 ?
3.4.22.61 acetyl-WEHD-p-nitroanilide + H2O 64% of the activity with acetyl-IETD-p-nitroanilide Cricetulus griseus acetyl-WEHD + p-nitroaniline
-
 ?
3.4.22.61 additional information caspase-8 is possibly involved in the apoptotic cell death in batch and fed-batch cultures of CHO cells Cricetulus griseus ?
-
 ?
3.4.22.62 acetyl-Ile-Glu-Thr-Asp-p-nitroanilide + H2O 13% of the activity with acetyl-Leu-Glu-His-Asp-p-nitroanilide Cricetulus griseus acetyl-Ile-Glu-Thr-Asp + p-nitroaniline
-
 ?
3.4.22.62 acetyl-Leu-Glu-His-Asp-p-nitroanilide + H2O
-
 Cricetulus griseus acetyl-Leu-Glu-His-Asp + p-nitroaniline
-
 ?
3.4.22.62 acetyl-Trp-Glu-His-Asp-p-nitroanilide + H2O 49% of the activity with acetyl-Leu-Glu-His-Asp-p-nitroanilide Cricetulus griseus acetyl-Trp-Glu-His-Asp + p-nitroaniline
-
 ?
3.4.22.62 acetyl-Val-Glu-Ile-Asp-p-nitroanilide + H2O 19% of the activity with acetyl-Leu-Glu-His-Asp-p-nitroanilide Cricetulus griseus acetyl-Val-Glu-Ile-Asp + p-nitroaniline
-
 ?
3.4.22.62 additional information caspase-9 is possibly involved in the apoptotic cell death in batch and fed-batch cultures of CHO cells Cricetulus griseus ?
-
 ?
3.4.22.62 additional information no activity with acetyl-Asp-Glu-Val-Asp-p-nitroanilide and acetyl-Val-Asp-Val-Ala-Asp-p-nitroanilide Cricetulus griseus ?
-
 ?