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Literature summary extracted from

  • Chong, P.K.; Burja, A.M.; Radianingtyas, H.; Fazeli, A.; Wright, P.C.
    Proteome and transcriptional analysis of ethanol-grown Sulfolobus solfataricus P2 reveals ADH2, a potential alcohol dehydrogenase (2007), J. Proteome Res., 6, 3985-3994.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expressed in Escherichia coli BL21 Star (DE3) cells Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.19
-
 n-Propanol 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C Saccharolobus solfataricus
1.1.1.1 0.44
-
 benzylalcohol 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C Saccharolobus solfataricus
1.1.1.1 1.33
-
 ethanol 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C Saccharolobus solfataricus
1.1.1.1 2.4
-
 iso-propanol 2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zn2+ ADH is a putative zinc-dependent alcohol dehydrogenase Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 36000
-
 deduced from amino acid sequence Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharolobus solfataricus Q9UXF1
-
-
1.1.1.1 Saccharolobus solfataricus P2 Q9UXF1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 HIS-Select High Flow cartridge chromatography Saccharolobus solfataricus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 culture condition:ethanol-grown cell highest ethanol consumption rate in cultures grown on 0.79% w/v ethanol Saccharolobus solfataricus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 0.03
-
 crude extract, using benzylalcohol as substrate, at 65°C Saccharolobus solfataricus
1.1.1.1 0.18
-
 after 5.67fold purification, using benzylalcohol as substrate, at 65°C Saccharolobus solfataricus
1.1.1.1 0.75
-
 purified enzyme, using benzylalcohol as substrate, at 80°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 benzylalcohol + NAD+
-
 Saccharolobus solfataricus benzaldehyde + NADH + H+
-
 ?
1.1.1.1 benzylalcohol + NAD+
-
 Saccharolobus solfataricus P2 benzaldehyde + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+
-
 Saccharolobus solfataricus acetaldehyde + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+
-
 Saccharolobus solfataricus P2 acetaldehyde + NADH + H+
-
 ?
1.1.1.1 iso-propanol + NAD+
-
 Saccharolobus solfataricus isopropanal + NADH + H+
-
 ?
1.1.1.1 iso-propanol + NAD+
-
 Saccharolobus solfataricus P2 isopropanal + NADH + H+
-
 ?
1.1.1.1 n-propanol + NAD+
-
 Saccharolobus solfataricus propanal + NADH + H+
-
 ?
1.1.1.1 n-propanol + NAD+
-
 Saccharolobus solfataricus P2 propanal + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH2
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 95
-
 activity increases with temperature up to 95°C Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Saccharolobus solfataricus