Literature summary extracted from

Lukacs, A.; Eker, A.P.; Byrdin, M.; Villette, S.; Pan, J.; Brettel, K.; Vos, M.H.
Role of the middle residue in the triple tryptophan electron transfer chain of DNA photolyase: ultrafast spectroscopy of a Trp-->Phe mutant (2006), J. Phys. Chem. B, 110, 15654-15658.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.1.99.3	cloned in Escherichia coli	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.99.3	Escherichia coli	P00914	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.1.99.3	protein is purified by chromatography on heparin-Sepharose CL-6B resin by elution with a 0.1-1.1 M NaCl gradient in 0.01 M potassium phosphate pH 7.0, 10 mM 2-mercaptoethanol, followed by chromatography on SP-Sepharose Fast Flow resin eluted with a 0.04-0.3 M NaCl gradient. To minimize oxidation of the FAD chromophore during purification, solutions are flushed with and kept under nitrogen	Escherichia coli

Synonyms

EC Number	Synonyms	Comment	Organism
4.1.99.3	DNA photolyase	-	Escherichia coli

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
4.1.99.3	FADH2	results indicate that both charge recombination of the primary charge separation state FADH-W382 and electron transfer from W359 to W382 occur with time constants below 4 ps, considerably faster than the initial W382-FADH electron-transfer step.	Escherichia coli

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Release 2023.1 (January 2023)