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Literature summary extracted from

  • Dominguez de Maria, P.; Stillger, T.; Pohl, M.; Wallert, S.; Drauz, K.; Groeger, H.; Trauthwein, H.; Liese, A.
    Preparative enantioselective synthesis of benzoins and (R)-2-hydroxy-1-phenylpropanone using benzaldehyde lyase (2006), J. Mol. Catal. B, 38, 43-47.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.38 expressed in Escherichia coli Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Mg2+ dependent, only in the presence of both cofactors, 0.015 mM Mg2+ and thiamine diphosphate, a significant enzymatic stabilisation is achieved Pseudomonas fluorescens

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.2.38 dithiothreitol positive effect on the enzyme stability Pseudomonas fluorescens
4.1.2.38 DMSO 30% (v/v) is optimal for the enzyme activity and stability Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 Pseudomonas fluorescens
-
 strain biovar I
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 4-methoxybenzaldehyde
-
 Pseudomonas fluorescens (R)-1,2-di-(4-methoxy-phenyl)-2-hydroxy-ethan-1-one
-
 ?
4.1.2.38 benzaldehyde + acetaldehyde
-
 Pseudomonas fluorescens (R)-2-hydroxy-1-phenylpropanone
-
 ?
4.1.2.38 benzoin
-
 Pseudomonas fluorescens benzaldehyde + benzaldehyde
-
 r
4.1.2.38 furan-carbaldehyde
-
 Pseudomonas fluorescens (R)-1,2-difuryl-2-hydroxy-ethan-1-one
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.38 BAL
-
 Pseudomonas fluorescens
4.1.2.38 benzaldehyde lyase
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.38 9.5
-
 highest carboligation activity, 10fold increase in activity compared to pH 7.0 Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.38 10
-
 sharp decrease in activity above pH 10 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate dependent, only in the presence of both cofactors, 0.015 mM Mg2+ and thiamine diphosphate, a significant enzymatic stabilisation is achieved Pseudomonas fluorescens