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Literature summary extracted from

  • Gu, Z.; Rao, M.K.; Forsyth, W.R.; Finke, J.M.; Matthews, C.R.
    Structural analysis of kinetic folding intermediates for a TIM barrel protein, indole-3-glycerol phosphate synthase, by hydrogen exchange mass spectrometry and Go model simulation (2007), J. Mol. Biol., 374, 528-546.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.48
-
 Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.1.48 urea the structure of partially folded states of the enzyme is assessed by hydrogen exchange mass spectrometry and Gรถ model simulations. HX-MS analysis of the peptic peptides derived from the pulse-labeled product of the submillisecond folding reaction from the urea-denatured state reveal strong protection in the (betaalpha)4 region, modest protection in the neighboring (betaalpha)1ย–3 and (betaalpha)5beta6 segments and no significant protection in the remaining N and C-terminal segments. The results demonstrate that this species is not a collapsed form of the unfolded state under native-favoring conditions nor is it the native state formed via fast-track folding Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Saccharolobus solfataricus
-
-
-
4.1.1.48 Saccharolobus solfataricus Q06121
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.48
-
 Saccharolobus solfataricus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.1.1.48 initiated by rapid 11fold dilution in refolding buffer (10 mM phosphate pH 7.8 and 25ยฐC) Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Saccharolobus solfataricus 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.48 IGPS
-
 Saccharolobus solfataricus
4.1.1.48 indole-3-glycerol phosphate synthase
-
 Saccharolobus solfataricus