EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.122 | the structure is solved to a resolution of 1.80 A by single-wavelength anomalous diffraction and phased using the two endogenous zinc ions per subunit. The structure reveals a catalytic and cofactor binding domain | Saccharolobus solfataricus |
1.1.1.427 | the structure is solved to a resolution of 1.80 A by single-wavelength anomalous diffraction and phased using the two endogenous zinc ions per subunit. The structure reveals a catalytic and cofactor binding domain | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.427 | 0.038 | - |
NADP+ | pH 7.5, 70°C, determined with 10 mM D-arabinose | Saccharolobus solfataricus | |
1.1.1.427 | 0.22 | - |
L-fucose | pH 7.5, 70°C, determined with 0.4 mM NADP+ | Saccharolobus solfataricus | |
1.1.1.427 | 1.25 | - |
NAD+ | pH 7.5, 70°C, determined with 10 mM D-arabinose | Saccharolobus solfataricus | |
1.1.1.427 | 1.4 | - |
D-arabinose | pH 7.5, 70°C, determined with 0.4 mM NADP+ | Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.122 | Zn2+ | contains two endogenous zinc ions per subunit.A zinc ion is bound in the loop that protrudes from the catalytic domain. This zinc is tetrahedrally coordinated by residues D94, C97, C100 and C108 | Saccharolobus solfataricus | |
1.1.1.427 | Zn2+ | contains two endogenous zinc ions per subunit.A zinc ion is bound in the loop that protrudes from the catalytic domain. This zinc is tetrahedrally coordinated by residues D94, C97, C100 and C108 | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.122 | Saccharolobus solfataricus | Q97YM2 | - |
- |
1.1.1.122 | Saccharolobus solfataricus P2 | Q97YM2 | - |
- |
1.1.1.427 | Saccharolobus solfataricus | Q97YM2 | - |
- |
1.1.1.427 | Saccharolobus solfataricus P2 | Q97YM2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.427 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.122 | 6-deoxy-L-galactose + NAD+ | i.e. L-fucose. The enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. This configuration is shared among the hexoses L-fucose (6-deoxy-L-galactose) and L-galactose, and the pentose D-ribose. Although the highest activity is observed with L-fucose in combination with NAD+, the remaining sugars are oxidized at higher rates using NADP+ | Saccharolobus solfataricus | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
1.1.1.122 | 6-deoxy-L-galactose + NAD+ | i.e. L-fucose. The enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. This configuration is shared among the hexoses L-fucose (6-deoxy-L-galactose) and L-galactose, and the pentose D-ribose. Although the highest activity is observed with L-fucose in combination with NAD+, the remaining sugars are oxidized at higher rates using NADP+ | Saccharolobus solfataricus P2 | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
1.1.1.427 | D-arabinose + NAD+ | - |
Saccharolobus solfataricus | D-arabinono-1,4-lactone + NADH + H+ | - |
? | |
1.1.1.427 | D-arabinose + NAD+ | - |
Saccharolobus solfataricus P2 | D-arabinono-1,4-lactone + NADH + H+ | - |
? | |
1.1.1.427 | D-arabinose + NADP+ | - |
Saccharolobus solfataricus | D-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.427 | D-arabinose + NADP+ | - |
Saccharolobus solfataricus P2 | D-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.427 | D-ribose + NADP+ | - |
Saccharolobus solfataricus | D-ribonolactone + NADPH | - |
? | |
1.1.1.427 | L-fucose + NADP+ | - |
Saccharolobus solfataricus | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.427 | L-fucose + NADP+ | - |
Saccharolobus solfataricus P2 | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.427 | L-galactose + NADP+ | - |
Saccharolobus solfataricus | L-galactonolactone + NADPH | - |
? | |
1.1.1.427 | additional information | the enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. Negligible activity with D-glucose, D-galactose, D-fucose, L-arabinose and D-xylose | Saccharolobus solfataricus | ? | - |
? | |
1.1.1.427 | additional information | the enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. Negligible activity with D-glucose, D-galactose, D-fucose, L-arabinose and D-xylose | Saccharolobus solfataricus P2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.122 | homotetramer | - |
Saccharolobus solfataricus |
1.1.1.427 | homotetramer | - |
Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.427 | AraDH | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.427 | 70 | - |
assay at | Saccharolobus solfataricus |
1.1.1.427 | 91 | - |
- |
Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.427 | 70 | 100 | 70°C: about 40% of maximal activity, 100°C: about 90% of maximal activity | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.427 | 85 | - |
half-life: 42 min | Saccharolobus solfataricus |
1.1.1.427 | 90 | - |
half-life: 26 min | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.427 | 7.5 | - |
assay at | Saccharolobus solfataricus |
1.1.1.427 | 8.2 | - |
- |
Saccharolobus solfataricus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.427 | 7.1 | 9.1 | pH 7.1: about 40% of maximal activity, pH 9.1: about 60% of maximal activity, pH 9.5: about 30% of maximal activity | Saccharolobus solfataricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.122 | NAD+ | the enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. This configuration is shared among the hexoses L-fucose (6-deoxy-L-galactose) and L-galactose, and the pentose D-ribose. Although the highest activity is observed with L-fucose in combination with NAD+, the remaining sugars are oxidized at higher rates using NADP+ | Saccharolobus solfataricus | |
1.1.1.427 | NAD+ | the catalytic efficiency of NADP+ in combination with D-arabinose is 36-fold higher than that of NAD+ | Saccharolobus solfataricus | |
1.1.1.427 | NADP+ | the catalytic efficiency of NADP+ in combination with D-arabinose is 36-fold higher than that of NAD+ | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.427 | 10.6 | - |
D-arabinose | pH 7.5, 70°C, determined with 0.4 mM NADP+ | Saccharolobus solfataricus | |
1.1.1.427 | 14.5 | - |
NAD+ | pH 7.5, 70°C, determined with 10 mM D-arabinose | Saccharolobus solfataricus | |
1.1.1.427 | 67 | - |
L-fucose | pH 7.5, 70°C, determined with 0.4 mM NADP+ | Saccharolobus solfataricus | |
1.1.1.427 | 525 | - |
NADP+ | pH 7.5, 70°C, determined with 10 mM D-arabinose | Saccharolobus solfataricus |