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Literature summary extracted from

  • Stengl, B.; Meyer, E.A.; Heine, A.; Brenk, R.; Diederich, F.; Klebe, G.
    Crystal structures of tRNA-guanine transglycosylase (TGT) in complex with novel and potent inhibitors unravel pronounced induced-fit adaptations and suggest dimer formation upon substrate binding (2007), J. Mol. Biol., 370, 492-511.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.29 medicine the enzyme is a target for the design of potent drugs against Shigellosis Zymomonas mobilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.29 enzyme-inhibitor complexes with 3,5-diaminophthalhydrazide, 4-aminophthalhydrazide, 5-aminonaphthalene-2,3-hydrazide, 5-amino-3-(1H-[1,2,4]triazole-3-yl-sulfanyl)-phthalhydrazide, 5-amino-3-(5-amino-1H-[1,2,4]triazol-3-yl-sulfanyl)-phthalhydrazide. 1.95 A crystal structure of 4-aminophthalhydrazide in complex with the enzyme Zymomonas mobilis
2.4.2.29 in complex with linbenzoguanine (6-aminoimidazol[4,5-g]quinazolin-8(7H)-one), hanging drop vapour diffusion method, in with 100 mM MES buffer (pH 5.5), 1 mM dithiothreitol, 8% (w/v) PEG 8000, 10% DMSO, at 20°C Zymomonas mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.29 2,3-Dihydroxybenzoic acid
-
 Zymomonas mobilis
2.4.2.29 2,6-diamino-8-(2-phenylethyl)quinazolin-4(3H)-one
-
 Zymomonas mobilis
2.4.2.29 2,6-diaminoquinazolin-4(3H)-one
-
 Zymomonas mobilis
2.4.2.29 2-amino-8-methylquinazolin-4(3H)-one
-
 Zymomonas mobilis
2.4.2.29 2-aminoquinazolin-4(3H)-one
-
 Zymomonas mobilis
2.4.2.29 3,5-diaminophthalhydrazide
-
 Zymomonas mobilis
2.4.2.29 3,5-diaminophthalimide
-
 Zymomonas mobilis
2.4.2.29 4-aminophthalhydrazide
-
 Zymomonas mobilis
2.4.2.29 4-aminophthalimide
-
 Zymomonas mobilis
2.4.2.29 5-amino-3-(1H-[1,2,4]triazole-3-yl-sulfanyl)-phthalhydrazide
-
 Zymomonas mobilis
2.4.2.29 5-amino-3-(5-amino-1H-[1,2,4]triazol-3-yl-sulfanyl)-phthalhydrazide
-
 Zymomonas mobilis
2.4.2.29 6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-4-[2-(4-methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
 Zymomonas mobilis
2.4.2.29 6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
 Zymomonas mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.29 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine Zymomonas mobilis
-
 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.29 Zymomonas mobilis
-
-
-
2.4.2.29 Zymomonas mobilis P28720
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.29 guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
-
 Zymomonas mobilis 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.29 dimer x-ray crystallography Zymomonas mobilis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.29 QueTGT
-
 Zymomonas mobilis
2.4.2.29 TGT
-
 Zymomonas mobilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.29 0.0002
-
 3,5-diaminophthalhydrazide
-
 Zymomonas mobilis
2.4.2.29 0.00048
-
 4-aminophthalimide
-
 Zymomonas mobilis
2.4.2.29 0.001
-
 2-amino-8-methylquinazolin-4(3H)-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.0015
-
 2,6-diaminoquinazolin-4(3H)-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.0021
-
 3,5-diaminophthalimide
-
 Zymomonas mobilis
2.4.2.29 0.0021
-
 2-aminoquinazolin-4(3H)-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.0026
-
 2,6-diamino-8-(2-phenylethyl)quinazolin-4(3H)-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.0037
-
 6-amino-4-[2-(4-methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.0041
-
 (6-aminoimidazol[4,5-g]quinazolin-8(7H)-one) competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.0069
-
 6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.0073
-
 6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.0083
-
 4-aminophthalhydrazide
-
 Zymomonas mobilis
2.4.2.29 0.015
-
 6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.029
-
 6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one competitive inhibition, in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 0.038
-
 5-amino-3-(1H-[1,2,4]triazole-3-yl-sulfanyl)-phthalhydrazide
-
 Zymomonas mobilis
2.4.2.29 0.05
-
 6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one in 200 mM HEPES buffer (pH 7.3), 20 mM MgCl2 Zymomonas mobilis
2.4.2.29 3
-
 2,3-Dihydroxybenzoic acid
-
 Zymomonas mobilis