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Literature summary extracted from

  • Kim, S.G.; Cavalier, M.; El-Maghrabi, M.R.; Lee, Y.H.
    A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (2007), J. Mol. Biol., 370, 14-26.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.46 overexpression in Escherichia coli as His6-tagged fusion protein Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.46 by the sitting-drop, vapor-diffusion method, crystals of PFKFB D-fructose 6-phosphate complex in the presence of the nonreactive ATP-analogue AMPPCP beta,gamma-methyleneadenosine 5’-triphosphate and crystals the inhibitory complex of PFKFB ADP and phosphoenolpyruvate Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.46 A44G site directed mutagenesis Homo sapiens
3.1.3.46 A44V site directed mutagenesis Homo sapiens
3.1.3.46 K168A site directed mutagenesis Homo sapiens
3.1.3.46 K168N site directed mutagenesis Homo sapiens
3.1.3.46 K168R site directed mutagenesis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.46 phosphoenolpyruvate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.46 0.0102
-
 beta-D-fructose 2,6-bisphosphate wild type enzyme Homo sapiens
3.1.3.46 0.038
-
 beta-D-fructose 2,6-bisphosphate A144G mutant Homo sapiens
3.1.3.46 0.204
-
 beta-D-fructose 2,6-bisphosphate A144V mutant Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.46 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.46 using Ni-NTA affinity columns Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate in-line phosphoryl transfer initiated by a substrate-assisted mechanism Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O
-
 Homo sapiens D-fructose 6-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.46 6-phosphofructo-2-kinase/fructose-2 6-biphosphatase
-
 Homo sapiens
3.1.3.46 PFKFB3
-
 Homo sapiens