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Literature summary extracted from

  • Jackson, C.J.; Carr, P.D.; Liu, J.W.; Watt, S.J.; Beck, J.L.; Ollis, D.L.
    The structure and function of a novel glycerophosphodiesterase from Enterobacter aerogenes (2007), J. Mol. Biol., 367, 1047-1062.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.2 expression in Escherichia coli Klebsiella aerogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.2
-
 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.2 Co2+
-
 Klebsiella aerogenes
3.1.4.2 Zn2+ activity of enzyme with Zn2+ 30 fold less than with Co2+ with substrate bis(p-nitro-phenyl)phosphate Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.2 30800
-
 monomer Klebsiella aerogenes
3.1.4.2 185700
-
 electrospray ionisation mass spectrometry, under non-denaturing conditions, consistent with calculated mass of hexamer Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.2 glycero-3-phosphoethanolamine + H2O Klebsiella aerogenes hydrolysis of the 3'-5' phosphodiester bond sn-glycerol-3-phosphate + ethanolamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.2 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.2 gel filtration Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.2 bis(p-nitro-phenyl)phosphate + H2O
-
 Klebsiella aerogenes ?
-
 ?
3.1.4.2 glycero-3-phosphoethanolamine + H2O hydrolysis of the 3'-5' phosphodiester bond Klebsiella aerogenes sn-glycerol-3-phosphate + ethanolamine
-
 ?
3.1.4.2 glycero-3-phosphoethanolamine + H2O also active on phosphomonoester, phosphodiester, phosphotriester and phosphorothiolate substrates Klebsiella aerogenes sn-glycerol-3-phosphate + ethanolamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.2 hexamer three dimers stabilized by an inter-chain disulfide bond, alpha/beta-sandwich Klebsiella aerogenes

Synonyms

EC Number Synonyms Comment Organism
3.1.4.2 glycerophosphodiesterase
-
 Klebsiella aerogenes
3.1.4.2 GpdQ
-
 Klebsiella aerogenes