EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.99.3 | cloned in Escherichia coli as a C-terminal His-tagged fusion protein | Sulfurisphaera tokodaii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.99.3 | crystal structure of the protein is determined at 2.8 A resolution with the molecular replacement method. Crystal structure of the St-photolyase is superimposed very well on the three known photolyases from Escherichia coli, Anacystis nidulans and Thermus thermophilus including the catalytic cofactor FAD. Surprisingly, another FAD molecule is found at the position of the light-harvesting cofactor. This second FAD molecule is well accommodated in the crystal structure, suggesting that FAD works as a novel light-harvesting cofactor of photolyase. In addition, two of the four CPD recognition residues in the crystal structure of Anacystis nidulans are not found in St-photolyase, indicating that St-photolyase might recognize cyclobutane pyrimidine dimer with a geometry different from that of Anphotolyase. RCSB PDB accession code: 2E0I | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.3 | Sulfurisphaera tokodaii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.99.3 | protein is purified using Ni-NTA and hydrophobic columns | Sulfurisphaera tokodaii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.99.3 | additional information | - |
protein shows phoreactive activity determined by UV/Vis-spectroscopy at 265 nm | Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.3 | cyclobutadipyrimidine in DNA | - |
Sulfurisphaera tokodaii | 2 pyrimidine residues in DNA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.3 | photolyase | - |
Sulfurisphaera tokodaii |
4.1.99.3 | St-photolyase | - |
Sulfurisphaera tokodaii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.3 | FAD | - |
Sulfurisphaera tokodaii |