Literature summary extracted from
Yamada, S.; Akiyama, S.; Sugimoto, H.; Kumita, H.; Ito, K.; Fujisawa, T.; Nakamura, H.; Shiro, Y.
The signaling pathway in histidine kinase and the response regulator complex revealed by X-ray crystallography and solution scattering (2006), J. Mol. Biol., 362, 123-139.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.7.13.3 |
additional information |
responsible role of the response regulator TrrA for the feedback regulation of sensing and/or kinase activities of ThkA |
Thermotoga maritima |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.13.3 |
expressed in Escherichia coli BL21 (DE3) CodonPlus RIL-X |
Thermotoga maritima |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.13.3 |
ThkA complexed with the response regulator TrrA, by the batch method, X-ray crystallography at a resolution of 4.2 A, 2fold symmetry, two monomeric TrrAs bind to the ThkA dimer |
Thermotoga maritima |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.13.3 |
additional information |
delta408ThkA, includes the PAS-sensor, the DHp and CA domains, and delta 517ThkA without the PAS-sensor domain, both are fully active in the autophosphorylation reaction |
Thermotoga maritima |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.13.3 |
51000 |
- |
delta517ThkA, experimental estimates |
Thermotoga maritima |
2.7.13.3 |
76000 |
- |
delta408ThkA, experimental estimates |
Thermotoga maritima |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.13.3 |
Thermotoga maritima |
Q9X180 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.13.3 |
by gel filtration |
Thermotoga maritima |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.13.3 |
additional information |
autophosphorylation activity |
Thermotoga maritima |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.13.3 |
dimer |
crystallography |
Thermotoga maritima |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.13.3 |
histidine kinase |
- |
Thermotoga maritima |
2.7.13.3 |
ThkA |
- |
Thermotoga maritima |