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Literature summary extracted from

  • Balk, S.H.; Yoshioka, H.; Yukawa, H.; Harayama, S.
    Synthesis of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) with thermostabilized low-specific L-threonine aldolase from Streptomyces coelicolor A3(2) (2007), J. Microbiol. Biotechnol., 17, 721-727.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.48 expressed in Escherichia coli strain JM109 Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.48 A169T stability-enhanced mutant, half life at 63°C is 3.7 min Streptomyces coelicolor
4.1.2.48 D104N stability-enhanced mutant, half life at 63°C is 5.8 min Streptomyces coelicolor
4.1.2.48 F18I stability-enhanced mutant, half life at 63°C is 5.0 min, the specific activity is decreased by 45% compared to the wild type enzyme Streptomyces coelicolor
4.1.2.48 H177Y stability-enhanced mutant, half life at 63°C is 14.6 min Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.48 0.000002
-
 L-phenylserine recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.0000026
-
 L-phenylserine mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.00016
-
 L-Thr mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.00018
-
 L-Thr recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.0002
-
 L-threo-3,4-dihydroxyphenylserine recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.00023
-
 L-threo-3,4-dihydroxyphenylserine mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.48 37000
-
 subunit, SDS-PAGE Streptomyces coelicolor
4.1.2.48 37500
-
 4 * 37500, gel filtration Streptomyces coelicolor
4.1.2.48 150000
-
 gel filtration Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.48 Streptomyces coelicolor
-
-
-
4.1.2.48 Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.48 Hiprep 16/10 DEAE FF column chromatography, Super Sepharose column chromatography, and HiLoad 16/10 Phenyl Sepharose HP column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.48 glycine + 3,4-dihydroxybenzaldehyde L-threo-3,4-dihydroxyphenylserine synthesis activity is dramatically decreased when the condensation reaction is repeated Streptomyces coelicolor L-threo-3,4-dihydroxyphenylserine
-
 ?
4.1.2.48 glycine + 3,4-dihydroxybenzaldehyde L-threo-3,4-dihydroxyphenylserine synthesis activity is dramatically decreased when the condensation reaction is repeated Streptomyces coelicolor A3(2) L-threo-3,4-dihydroxyphenylserine
-
 ?
4.1.2.48 L-phenylserine
-
 Streptomyces coelicolor ?
-
 ?
4.1.2.48 L-phenylserine
-
 Streptomyces coelicolor A3(2) ?
-
 ?
4.1.2.48 L-Thr
-
 Streptomyces coelicolor glycine + acetaldehyde
-
 ?
4.1.2.48 L-Thr
-
 Streptomyces coelicolor A3(2) glycine + acetaldehyde
-
 ?
4.1.2.48 L-threo-3,4-dihydroxyphenylserine
-
 Streptomyces coelicolor glycine + 3,4-dihydroxybenzaldehyde
-
 ?
4.1.2.48 L-threo-3,4-dihydroxyphenylserine
-
 Streptomyces coelicolor A3(2) glycine + 3,4-dihydroxybenzaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.48 homotetramer 4 * 37500, gel filtration Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
4.1.2.48 L-TA
-
 Streptomyces coelicolor
4.1.2.48 L-threonine aldolase
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.48 50
-
 recombinant enzyme Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.48 60
-
 the residual L-TA activity after a heat treatment for 20 min at 60°C is 10.6% Streptomyces coelicolor
4.1.2.48 63
-
 the half-life of the wild type L-TA at 63°C is 1.3 min Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.48 0.000107
-
 L-phenylserine recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.000113
-
 L-phenylserine mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.00022
-
 L-threo-3,4-dihydroxyphenylserine recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.00023
-
 L-threo-3,4-dihydroxyphenylserine mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.0178
-
 L-Thr mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor
4.1.2.48 0.0195
-
 L-Thr recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.48 7 8 recombinant enzyme Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.48 pyridoxal 5'-phosphate activates Streptomyces coelicolor