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Literature summary extracted from

  • Zhang, J.; Lin, S.; Zeng, R.
    Cloning, expression, and characterization of a cold-adapted lipase gene from an antarctic deep-sea psychrotrophic bacterium, Psychrobacter sp. 7195 (2007), J. Microbiol. Biotechnol., 17, 604-610.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 CHAPS
-
 Psychrobacter sp.
3.1.1.3 additional information effects of detergents on LipA1 activity, overview Psychrobacter sp.
3.1.1.3 SDS
-
 Psychrobacter sp.
3.1.1.3 Span 40
-
 Psychrobacter sp.
3.1.1.3 Span 60
-
 Psychrobacter sp.
3.1.1.3 Triton X-100
-
 Psychrobacter sp.
3.1.1.3 Tween 40
-
 Psychrobacter sp.
3.1.1.3 Tween 80
-
 Psychrobacter sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 gene lipA1, DNA and amino acid sequence determination and analysis, screening a genomic DNA library, expression of LipA1 in Escherichia coli strain BL21 (DE3) Psychrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Cd2+
-
 Psychrobacter sp.
3.1.1.3 Co2+
-
 Psychrobacter sp.
3.1.1.3 EDTA
-
 Psychrobacter sp.
3.1.1.3 Fe2+
-
 Psychrobacter sp.
3.1.1.3 Fe3+
-
 Psychrobacter sp.
3.1.1.3 Hg2+
-
 Psychrobacter sp.
3.1.1.3 additional information effects of detergents on LipA1 activity, overview Psychrobacter sp.
3.1.1.3 Rb+
-
 Psychrobacter sp.
3.1.1.3 Zn2+
-
 Psychrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Psychrobacter sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ activates Psychrobacter sp.
3.1.1.3 Mg2+ activates Psychrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 35000
-
 x * 35210, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Psychrobacter sp.
3.1.1.3 35210
-
 x * 35210, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Psychrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Psychrobacter sp. Q2KTB5 psychrotrophic strain 7195 isolated from deepsea sediment of Prydz Bay, gene lipA1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant LipA1 35fold from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, gel filtration, and preparative polyacrylamide gel electrophoresis Psychrobacter sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate LipA1 is a cold-adapted enzyme and has one consensus motif GNSMG, containing the putative active-site serine, which is conserved in other cold-adapted lipolytic enzymes Psychrobacter sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 cell culture optimal and highest temperatures for growth of strain 7195 are 15°C and 35°C, respectively, exhibiting sigmoidal growth even at -3°C, no growth at 37°C or above Psychrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 1084
-
 purified recombinant enzyme Psychrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Psychrobacter sp. 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O high activity Psychrobacter sp. 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O best 4-nitrophenyl ester substrate Psychrobacter sp. 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O high activity Psychrobacter sp. 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl stearate + H2O
-
 Psychrobacter sp. 4-nitrophenol + stearate
-
 ?
3.1.1.3 additional information substrate specificity, LipA1 shows a preference for trimyristin and 4-nitrophenyl myristate, overview Psychrobacter sp. ?
-
 ?
3.1.1.3 olive oil + H2O high activity Psychrobacter sp. ?
-
 ?
3.1.1.3 triacetin + H2O
-
 Psychrobacter sp. diacetin + acetate
-
 ?
3.1.1.3 tributyrin + H2O
-
 Psychrobacter sp. dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O
-
 Psychrobacter sp. dicaprin + caprate
-
 ?
3.1.1.3 trimyristin + H2O best triacylglyceride substrate Psychrobacter sp. dimyristin + myristate
-
 ?
3.1.1.3 triolein + H2O
-
 Psychrobacter sp. diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O high activity Psychrobacter sp. dipalmitin + palmitate
-
 ?
3.1.1.3 tristearin + H2O
-
 Psychrobacter sp. distearin + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 35210, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Psychrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 LipA1
-
 Psychrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 30
-
-
 Psychrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3
-
 50 purified enzyme, substrate 4-nitrophenyl palmitate, maximal activity at 30°C, 18% and 35% of maximal activity at 0°C and 10°C, respectively Psychrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 10
-
 purified LipA1, completely stable up to Psychrobacter sp.
3.1.1.3 30
-
 purified LipA1, 5 h, 50% remaining activity Psychrobacter sp.
3.1.1.3 60
-
 purified LipA1, 15 min 50% remaining activity Psychrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9
-
-
 Psychrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 7 10 purified enzyme, 24 h, 0°C, 90% reamining activity Psychrobacter sp.