Literature summary extracted from

  • Seo, J.S.; An, J.H.; Baik, M.Y.; Park, C.S.; Cheong, J.J.; Moon, T.W.; Park, K.H.; Choi, Y.D.; Kim, C.H.
    Molecular cloning and characterization of trehalose biosynthesis genes from hyperthermophilic archaebacterium Metallosphaera hakonesis (2007), J. Microbiol. Biotechnol., 17, 123-129.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.141 additional information addition of 4-alpha-glucanotransferase increases the yield of trehalose production from maltooligosaccharide substrates supplying longer maltooligosaccharides that may be better substrates for the sequential reaction catalyzed by MTS and MTH Metallosphaera hakonensis
3.2.1.141 IPTG transcription induced by 1 mM Metallosphaera hakonensis

Application

EC Number Application Comment Organism
5.4.99.15 synthesis production of trehalose by enzyme plus maltooligosyltrehalose trehalohydrolase is increased in presence of 4-alpha-glucanotransferase, converting 70% of maltopentaose into trehalose after 6 h, while 60% is converted without 4-alpha-glucanotransferase Metallosphaera hakonensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.141 DNA construct pRBMhMTH expressed in Escherichia coli BL21(DE3)pLysS Metallosphaera hakonensis
5.4.99.15 expression in Escherichia coli Metallosphaera hakonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.15 90000
-
x * 83800, calculated, x * 90000, SDS-PAGE Metallosphaera hakonensis
3.2.1.141 63700
-
sequence analysis Metallosphaera hakonensis
5.4.99.15 83800
-
x * 83800, calculated, x * 90000, SDS-PAGE Metallosphaera hakonensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.141 Metallosphaera hakonensis
-
JCM 8857
-
5.4.99.15 Metallosphaera hakonensis
-
-
-
3.2.1.141 Metallosphaera hakonensis JCM 8857
-
JCM 8857
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.141 by Ni2+-NTA-agarose affinity chromatography Metallosphaera hakonensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.15 Maltopentaose
-
Metallosphaera hakonensis alpha-Maltotriosyl trehalose
-
?
5.4.99.15 Maltopentaose
-
Metallosphaera hakonensis JCM 8857 alpha-Maltotriosyl trehalose
-
?
3.2.1.141 maltotriosyltrehalose + H2O
-
Metallosphaera hakonensis maltotriose + alpha,alpha'-trehalose
-
?
3.2.1.141 maltotriosyltrehalose + H2O
-
Metallosphaera hakonensis JCM 8857 maltotriose + alpha,alpha'-trehalose
-
?
5.4.99.15 liquified corn starch
-
Metallosphaera hakonensis ? in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose ?
5.4.99.15 liquified corn starch
-
Metallosphaera hakonensis JCM 8857 ? in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose ?
3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase Metallosphaera hakonensis (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
?
3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase Metallosphaera hakonensis JCM 8857 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
?
5.4.99.15 soluble starch
-
Metallosphaera hakonensis ? in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose ?
5.4.99.15 soluble starch
-
Metallosphaera hakonensis JCM 8857 ? in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose ?

Subunits

EC Number Subunits Comment Organism
5.4.99.15 ? x * 83800, calculated, x * 90000, SDS-PAGE Metallosphaera hakonensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.141 Maltooligosyl trehalose trehalohydrolase
-
Metallosphaera hakonensis
3.2.1.141 MTH
-
Metallosphaera hakonensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.141 70
-
-
Metallosphaera hakonensis
5.4.99.15 70
-
-
Metallosphaera hakonensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.141 70
-
retainis 80% of the activity for 48 h, activity is gradually abolished at above 85°C Metallosphaera hakonensis
5.4.99.15 70
-
48 h, 80% residual activity Metallosphaera hakonensis
5.4.99.15 85
-
activity is gradually abolished Metallosphaera hakonensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.141 5
-
-
Metallosphaera hakonensis
5.4.99.15 5
-
-
Metallosphaera hakonensis