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Literature summary extracted from
- Molecular cloning and characterization of trehalose biosynthesis genes from hyperthermophilic archaebacterium Metallosphaera hakonesis (2007), J. Microbiol. Biotechnol., 17, 123-129.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.141 | IPTG | transcription induced by 1 mM | Metallosphaera hakonensis |  |
| 3.2.1.141 | additional information | addition of 4-alpha-glucanotransferase increases the yield of trehalose production from maltooligosaccharide substrates supplying longer maltooligosaccharides that may be better substrates for the sequential reaction catalyzed by MTS and MTH | Metallosphaera hakonensis |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | synthesis | production of trehalose by enzyme plus maltooligosyltrehalose trehalohydrolase is increased in presence of 4-alpha-glucanotransferase, converting 70% of maltopentaose into trehalose after 6 h, while 60% is converted without 4-alpha-glucanotransferase | Metallosphaera hakonensis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.141 | DNA construct pRBMhMTH expressed in Escherichia coli BL21(DE3)pLysS | Metallosphaera hakonensis |
| 5.4.99.15 | expression in Escherichia coli | Metallosphaera hakonensis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 63700 | - |
sequence analysis | Metallosphaera hakonensis |
| 5.4.99.15 | 83800 | - |
x * 83800, calculated, x * 90000, SDS-PAGE | Metallosphaera hakonensis |
| 5.4.99.15 | 90000 | - |
x * 83800, calculated, x * 90000, SDS-PAGE | Metallosphaera hakonensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.141 | Metallosphaera hakonensis | - |
JCM 8857 | - |
| 3.2.1.141 | Metallosphaera hakonensis JCM 8857 | - |
JCM 8857 | - |
| 5.4.99.15 | Metallosphaera hakonensis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.141 | by Ni2+-NTA-agarose affinity chromatography | Metallosphaera hakonensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.141 | 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase | Metallosphaera hakonensis | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
| 3.2.1.141 | 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase | Metallosphaera hakonensis JCM 8857 | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
| 3.2.1.141 | maltotriosyltrehalose + H2O | - |
Metallosphaera hakonensis | maltotriose + alpha,alpha'-trehalose | - |
? | |
| 3.2.1.141 | maltotriosyltrehalose + H2O | - |
Metallosphaera hakonensis JCM 8857 | maltotriose + alpha,alpha'-trehalose | - |
? | |
| 5.4.99.15 | liquified corn starch | - |
Metallosphaera hakonensis | ? | in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose | ? | |
| 5.4.99.15 | liquified corn starch | - |
Metallosphaera hakonensis JCM 8857 | ? | in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose | ? | |
| 5.4.99.15 | Maltopentaose | - |
Metallosphaera hakonensis | alpha-Maltotriosyl trehalose | - |
? | |
| 5.4.99.15 | Maltopentaose | - |
Metallosphaera hakonensis JCM 8857 | alpha-Maltotriosyl trehalose | - |
? | |
| 5.4.99.15 | soluble starch | - |
Metallosphaera hakonensis | ? | in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose | ? | |
| 5.4.99.15 | soluble starch | - |
Metallosphaera hakonensis JCM 8857 | ? | in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | ? | x * 83800, calculated, x * 90000, SDS-PAGE | Metallosphaera hakonensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.141 | Maltooligosyl trehalose trehalohydrolase | - |
Metallosphaera hakonensis |
| 3.2.1.141 | MTH | - |
Metallosphaera hakonensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 70 | - |
- |
Metallosphaera hakonensis |
| 5.4.99.15 | 70 | - |
- |
Metallosphaera hakonensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 70 | - |
retainis 80% of the activity for 48 h, activity is gradually abolished at above 85°C | Metallosphaera hakonensis |
| 5.4.99.15 | 70 | - |
48 h, 80% residual activity | Metallosphaera hakonensis |
| 5.4.99.15 | 85 | - |
activity is gradually abolished | Metallosphaera hakonensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 5 | - |
- |
Metallosphaera hakonensis |
| 5.4.99.15 | 5 | - |
- |
Metallosphaera hakonensis |
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Release 2023.1 (January 2023)
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