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Literature summary extracted from

  • Choi, J.J.; Park, J.W.; Shim, H.; Lee, S.; Kwon, M.; Yang, J.; Hwang, H.; Kwon, S.
    Cloning, expression, and characterization of a hyperalkaline phosphatase from the thermophilic bacterium Thermus sp. T351 (2006), J. Microbiol. Biotechnol., 16, 272-279.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.1 expressed under the control of the T7lac promoter on the expression vector pET-22b(+) in Escherichia coli BL21(DE3) Thermus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 EDTA
-
 Thermus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.039
-
 4-nitrophenyl phosphate 80°C, pH 12 Thermus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Co2+ 1 mM, activates Thermus sp.
3.1.3.1 Mg2+ 1 mM, 10fold activation Thermus sp.
3.1.3.1 Mn2+ 1 mM, slight activation Thermus sp.
3.1.3.1 Zn2+ 1 mM, slight activation Thermus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 52000
-
 x * 52000, SDS-PAGE Thermus sp.
3.1.3.1 54407
-
 x * 54407, calculated from sequence Thermus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Thermus sp. B8K1W1
-
-
3.1.3.1 Thermus sp. T351 B8K1W1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 recombinant Thermus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 30.8
-
-
 Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 2'-deoxyadenosine 5'-monophosphate + H2O 113.3% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 2'-deoxyadenosine 5'-monophosphate + H2O 113.3% of the activity with 4-nitrophenyl phosphate Thermus sp. T351 ?
-
 ?
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Thermus sp. 4-nitrophenol + phosphate
-
 ?
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Thermus sp. T351 4-nitrophenol + phosphate
-
 ?
3.1.3.1 5-bromo-4-chloro-3-indolyl phosphate + H2O 50.3% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 5-bromo-4-chloro-3-indolyl phosphate + H2O 50.3% of the activity with 4-nitrophenyl phosphate Thermus sp. T351 ?
-
 ?
3.1.3.1 adenosine 5'-monophosphate + H2O 306.1% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 adenosine 5'-triphosphate + H2O 257.4% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 alpha-naphthyl acid phosphate + H2O 88.6% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 bis(p-nitrophenyl) phosphate + H2O 6.1% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 bis(p-nitrophenyl) phosphate + H2O 6.1% of the activity with 4-nitrophenyl phosphate Thermus sp. T351 ?
-
 ?
3.1.3.1 cytidine 5'-monophosphate + H2O 343.8% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 glycerophosphate + H2O 248.7% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 glycerophosphate + H2O 248.7% of the activity with 4-nitrophenyl phosphate Thermus sp. T351 ?
-
 ?
3.1.3.1 guanosine 5'-monophosphate + H2O 358.8% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?
3.1.3.1 uridine 5'-monophosphate + H2O 388.6% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 ? x * 52000, SDS-PAGE Thermus sp.
3.1.3.1 ? x * 54407, calculated from sequence Thermus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 80
-
-
 Thermus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 60 90 60°C: aout 50% of maximal activity, 90°C: about 40% of maximal activity Thermus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 85
-
 half-life is 103 min Thermus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 12
-
-
 Thermus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.1 7 12 25°C, 16 h, stable Thermus sp.